Novel approach to phasing proteins: derivatization by short cryo-soaking with halides

被引：274

作者：

Dauter, Z ^{[1
]}

Dauter, M ^{[1
]}

Rajashankar, KR ^{[1
]}

机构：

[1] NCI, SAIC, Frederick & Brookhaven Natl Lab, Upton, NY 11973 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444999016352

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A quick (less than 1 min) soak of protein crystals in a cryo-solution containing bromide or iodide anions leads to incorporation of these anomalous scatterers into the ordered solvent region around the protein molecules. These halide anions provide a convenient way of phasing through their anomalous scattering signal: bromides using multiwavelength anomalous dispersion (MAD) and bromides and/or iodides using single-wavelength anomalous dispersion (SAD) or single isomorphous replacement with anomalous scattering (SIRAS) methods. This approach has been tested successfully on four different proteins and has been used to solve the structure of a new protein of molecular weight 30 kDa.

引用

页码：232 / 237

页数：6

共 43 条

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