Massive lymphocyte apoptosis in the thymus of functionally deficient TrkB mice

被引：31

作者：

Garcia-Suarez, O

Blanco-Gelaz, MA

Lopez, ML

Germana, A

Cabo, R

Díaz-Esnal, B

Silos-Santiago, I

Ciriaco, E

Vega, JA

机构：

[1] Univ Oviedo, Dept Morfol & Biol Celular, Fac Med, Oviedo 33006, Spain

[2] Univ Messina, Dipartimento Morfol Biochim Fisiol & Prod Anim, Messina, Italy

[3] Cent Hosp, Serv Inmmunol, Oviedo, Spain

[4] Univ Oviedo, Dept Bioquim & Biol Mol, Oviedo, Spain

[5] Univ Valladolid, Dept Anat, Valladolid, Spain

[6] Millennium Pharmaceut Inc, Dept Neurobiol, Cambridge, MA USA

[7] Univ Oviedo, Inst Univ Oncol, Oviedo, Spain

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2002年 / 129卷 / 1-2期

关键词：

thymus; TrkB neurotrophin receptor; apoptosis; functionally deficient TrkB mice;

D O I：

10.1016/S0165-5728(02)00166-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The occurrence of TrkB in the murine thymus (15-day and 3-month old) was investigated by Northern blot, Western blot and immunohistochemistry. Furthermore, the thymus of 15-day-old mice carrying a non-functional mutation on trkB was analyzed. Both trkB mRNA and 145 kDa TrkB protein were detected. In addition, isolated lymphocytes and stromal cells also expressed this protein. The thymus of homozygous functionally TrkB-deficient animals showed structural and ultrastructural changes consistent with massive death of cortical lymphocytes, confirmed with TUNEL. Present results suggest a role for TrkB in maintaining the survival or preventing massive death of lymphocytes in the mammalian thymus. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：25 / 34

页数：10

共 41 条

[21] EXPRESSION OF MESSENGER-RNA ENCODING NEUROTROPHINS AND NEUROTROPHIN RECEPTORS IN RAT THYMUS, SPLEEN TISSUE AND IMMUNOCOMPETENT CELLS - REGULATION OF NEUROTROPHIN-4 MESSENGER-RNA EXPRESSION BY MITOGENS AND LEUKOTRIENE B-4 [J].