A conserved small GTP-binding protein Alp41 is essential for the cofactor-dependent biogenesis of microtubules in fission yeast

被引：49

作者：

Radcliffe, PA ^{[1
]}

Vardy, L ^{[1
]}

Toda, T ^{[1
]}

机构：

[1] Imperial Canc Res Fund, Lab Cell Regulat, London WC2A 3PX, England

来源：

FEBS LETTERS | 2000年 / 468卷 / 01期

关键词：

ARF-like protein; cell polarity; cofactor; GTP-binding protein; microtubule; fission yeast;

D O I：

10.1016/S0014-5793(00)01202-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The proper folding of tubulins and their incorporation into microtubules consist of a series of reactions. in which evolutionarily conserved proteins, cofactors A to E, play a vital role. We have cloned a fission east gene (alp41(+)) which encodes a highly conserved small GTP-binding protein homologous to budding yeast CIN4 and human ARF-like Arl2. alp41(+) is essential, disruption of which results in microtubule dysfunction and growth polarity defects. Genetic analysis indicates that Alp41 plays a crucial role in the cofactor-dependent pathway, in which it functions upstream of the cofactor D homologue Alp1(D) and possibly in concert with Alp21(E) (C) 2000 Federation of European Biochemical Societies.

引用

页码：84 / 88

页数：5

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