A Rapidly Maturing Far-Red Derivative of DsRed-Expres2 for Whole-Cell Labeling

被引：149

作者：

Strack, Rita L. ^{[2
]}

Hein, Birka ^{[3
]}

Bhattacharyya, Dibyendu ^{[1
]}

Hell, Stefan W. ^{[3
]}

Keenan, Robert J. ^{[2
]}

Glick, Benjamin S. ^{[1
]}

机构：

[1] Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA

[2] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA

[3] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37070 Gottingen, Germany

来源：

BIOCHEMISTRY | 2009年 / 48卷 / 35期

关键词：

GREEN FLUORESCENT PROTEIN; VARIANTS;

D O I：

10.1021/bi900870u

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescent proteins (FPs) with far-red excitation and emission are desirable for multicolor labeling and live-animal imaging. We describe E2-Crimson, a far-red derivative of the tetrameric FP DsRed-Express2. Unlike other far-red FPs, E2-Crimson is noncytotoxic in bacterial and mammalian cells. E2-Crimson is brighter than other far-red FPs and matures substantially faster than other red and far-red FPs. Approximately 40% of the E2-Crimson fluorescence signal is remarkably photostable. With an excitation maximum at 611 nm, E2-Crimson is the first FP that is efficiently excited with standard far-red lasers. We show that E2-Crimson has unique applications for flow cytometry and stimulated emission depletion (STED) microscopy.

引用

页码：8279 / 8281

页数：3

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