Strong augment effect of IL-12 expression plasmid on the induction of HIV-specific cytotoxic T lymphocyte activity by a peptide vaccine candidate

被引:21
作者
Hamajima, K
Fukushima, J
Bukawa, H
Kaneko, T
Tsuji, T
Asakura, Y
Sasaki, S
Xin, KQ
Okuda, K
机构
[1] YOKOHAMA CITY UNIV,SCH MED,DEPT ORAL & MAXILLOFACIAL SURG,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN
[2] YOKOHAMA CITY UNIV,SCH MED,DEPT INTERNAL MED 1,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN
来源
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1997年 / 83卷 / 02期
关键词
D O I
10.1006/clin.1997.4348
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We previously reported that repeated inoculation of VC1, a macromolecular multicomponent peptide vaccine emulsified with Freund's adjuvant (VC1-F), induced high cytotoxic T lymphocyte (CTL) levels and a substantial level of multivalent antibodies which neutralized various human immunodeficiency virus type 1 (HIV-1) isolates, In the present study, we report that inoculation of VC1-F plus interleukin (IL)-12 expression plasmid can induce a higher antigen-specific CTL response comparied to that with VC1-F alone. VC1-F plus IL-12 expression plasmid or VC1-F alone were inoculated to BALB/c mice twice at interval of 2 weeks. Two weeks after the second inoculation, spleen effector cells from these mice were examined. Stronger CTE responses against target cells were observed from the inoculation of VC1-F plus IL-12 plasmid than from that with VC-IF alone, but there was no difference in antibody induction. The inoculation of VC1 plus IL-12 plasmid also producted higher CTL activity than the inoculation of VC1 alone. These augmented CTL activities were not observed using target cells pulsed with non-HIV-specific peptides and different class I haplotype cells, These data demonstrate that co-inoculation of cell-mediated immune potent antigen and IL-12 plasmids can enhance the antigen-specific CTL response. This may be a potential approach for the induction of cellular immunization against HIV-1 and Other diseases. (C) 1997 Academic Press.
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页码:179 / 184
页数:6
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