Identifying the geographical origin of protected sea cucumbers (Apostichopus japonicus) in China using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

被引：25

作者：

Yun, Zhenyu ^{[1
]}

Sun, Zhao ^{[1
]}

Xu, Haiyan ^{[2
]}

Sun, Zhihong ^{[2
]}

Zhang, Yao ^{[1
]}

Liu, Zhen ^{[1
]}

机构：

[1] China Natl Inst Standardizat, Subinst Food & Agr Standardizat, Beijing 100191, Peoples R China

[2] Inner Mongolia Agr Univ, Coll Food Sci & Engn, Hohhot 010018, Inner Mongolia, Peoples R China

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2017年 / 26卷 / 02期

关键词：

sea cucumber; geographical origin; random amplified polymorphic DNA; genetic diversity; MARKERS;

D O I：

10.1007/s10068-017-0048-8

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Dalian sea cucumber, Yantai sea cucumber, and Weihai sea cucumber, which belong to Apostichopus japonicus, are protected as geographical indications in China based on their high nutritional values and medical propertys. The 26 samples, including Dalian sea cucumbers (9 samples) in Liaoning province, Yantai sea cucumbers (9 samples), and Weihai sea cucumbers (8 samples) in Shandong province, were individually collected from the designated geographical sea areas and the genetic relationships and DNA polymorphisms were evaluated by random amplified polymorphic DNA technology and gene segments sequencing. The RAPD dendrogram showed that the genetic diversity of the three types of sea cucumbers was rich. The neighbor-joining tree shows that the genetic relationship of the samples from the adjacent sea areas is closer. It demonstrates that the gene characteristics of sea cucumbers from different sea areas were obvious and the genetic diversity analysis by RAPD-PCR can be used as a rapid method for geographical discrimination.

引用

页码：357 / 362

页数：6

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