Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities

被引：82

作者：

Zhang, T ^{[1
]}

Fang, HHP ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Civil Engn, Ctr Environm Engn Res, Hong Kong, Hong Kong, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 05期

关键词：

biofilm; cluster analysis; DGGE; microbial community;

D O I：

10.1023/A:1005680803442

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA profiles were objectively digitized using an image analyzer; the individual microbial species in a community can thus be precisely quantified. The similarity between various microbial communities was compared to the digitized DGGE profiles using the cluster analyses technique. The microbial community in a biofilm was considerably different from that in suspended sludge obtained from the same system.

引用

页码：399 / 405

页数：7

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