Genome-Wide RNAi Screen Identifies Genes Involved in Intestinal Pathogenic Bacterial Infection

被引：236

作者：

Cronin, Shane J. F. ^{[2
]}

Nehme, Nadine T. ^{[1
]}

Limmer, Stefanie ^{[1
]}

Liegeois, Samuel ^{[1
]}

Pospisilik, J. Andrew ^{[2
]}

Schramek, Daniel ^{[2
]}

Leibbrandt, Andreas ^{[2
]}

Simoes, Ricardo de Matos ^{[3
]}

Gruber, Susanne ^{[2
]}

Puc, Urszula ^{[2
]}

Ebersberger, Ingo ^{[3
]}

Zoranovic, Tamara ^{[2
]}

Neely, G. Gregory ^{[2
]}

von Haeseler, Arndt ^{[3
]}

Ferrandon, Dominique ^{[1
]}

Penninger, Josef M. ^{[2
]}

机构：

[1] CNRS, Inst Biol Mol & Cellulaire, Equipe Fdn Rech Med, UPR 9022, F-67084 Strasbourg, France

[2] Austrian Acad Sci, Inst Mol Biotechnol, IMBA, A-1030 Vienna, Austria

[3] Univ Vet Med, Univ Vienna, Ctr Integrat Bioinformat CIBIV, Med Univ Vienna, A-1030 Vienna, Austria

来源：

SCIENCE | 2009年 / 325卷 / 5938期

基金：

奥地利科学基金会; 欧洲研究理事会;

关键词：

IMMUNE-RESPONSE; HOST-DEFENSE; STEM-CELLS; SERRATIA-MARCESCENS; IN-VIVO; DROSOPHILA; INTERFERENCE; ACTIVATION; AUTOPHAGY; VIRULENCE;

D O I：

10.1126/science.1173164

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity.

引用

页码：340 / 343

页数：4

共 34 条

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