A third base pair for the polymerase chain reaction: inserting isoC and isoG

被引：73

作者：

Johnson, SC ^{[1
]}

Sherrill, CB ^{[1
]}

Marshall, DJ ^{[1
]}

Moser, MJ ^{[1
]}

Prudent, JR ^{[1
]}

机构：

[1] EraGen Biosci Inc, Res Dept, Madison, WI 53717 USA

来源：

NUCLEIC ACIDS RESEARCH | 2004年 / 32卷 / 06期

关键词：

D O I：

10.1093/nar/gkh522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two additional bases (isoguanosine and isocytosine), generating a third base pair, have been implemented in PCR. Enzyme fidelity for the third base pair is demonstrated using molecular thermodynamic melting, chemical cleavage and molecular beacons. When amplifying as few as 15 targets containing multiple non-natural base pairs with 40 cycles of amplification, our results confirm sequence conservation. The additional sequence space provided by three base pairs allows for the construction of molecular tools that achieve higher complexity and better discrimination than those possible with natural DNA alone.

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页码：1937 / 1941

页数：5

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