Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples

被引：62

作者：

Makino, S ^{[1
]}

Kurazono, H

Chongsanguam, M

Hayashi, H

Cheun, H

Suzuki, S

Shirahata, T

机构：

[1] Obihiro Univ Agr & Vet Med, Dept Vet Microbiol, Obihiro, Hokkaido 0808555, Japan

[2] Okayama Univ, Sch Hlth Sci, Dept Med Technol, Okayama 7008558, Japan

[3] Univ Tsukuba, Inst Basic Med Sci, Dept Microbiol, Tsukuba, Ibaraki 3058557, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1999年 / 61卷 / 11期

关键词：

food; PCR; Salmonella;

D O I：

10.1292/jvms.61.1245

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We established the PCR detection system specific to Salmonella species using Salmonella enterotoxin gene (stn). The detection limit was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Tripticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public hygiene.

引用

页码：1245 / 1247

页数：3