Crosstalk with Cancer-Associated Fibroblasts Increases the Growth and Radiation Survival of Cervical Cancer Cells

被引：69

作者：

Chu, Tang-Yuan ^{[1
,2
,3
]}

Yang, June-Ting ^{[4
]}

Huang, Tien-Hung ^{[1
]}

Liu, Hwan-Wun ^{[5
]}

机构：

[1] Tzu Chi Univ, Inst Med Sci, Hualien 970, Taiwan

[2] Tzu Chi Univ, Buddhist Tzu Chi Gen Hosp, Dept Obstet & Gynecol, Hualien 970, Taiwan

[3] Buddhist Tzu Chi Gen Hosp, Dept Res, Ctr Cerv Canc Prevent, Hualien, Taiwan

[4] Tzu Chi Univ, Dept Mol Biol & Human Genet, Hualien 970, Taiwan

[5] Tzu Chi Univ, Buddhist Tzu Chi Gen Hosp, Dept Occupat Med, Hualien 970, Taiwan

来源：

RADIATION RESEARCH | 2014年 / 181卷 / 05期

关键词：

HELA-CELLS; FACTOR-IV; EXPRESSION; RADIOCHEMOTHERAPY; PROGRESSION; INHIBITORS; PATHWAY; BINDING; STRESS; EGFR;

D O I：

10.1667/RR13583.1

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Crosstalk between cancer cells and the surrounding cancer associated fibroblasts (CAFs) plays an illusive role in cancer radiotherapy. This study investigated the effect of cancer cell-cancer associated fibroblasts crosstalk on the proliferation and survival of irradiated cervical cancer cells. A pretreatment with conditioned medium from a mixed culture of CAF and HeLa cells (mixCAF) had a stronger effect on enhancing the proliferation and survival of irradiated HeLa cells compared to pretreatment with CAF conditioned medium alone. In addition, pretreatment with a mixed culture of CAF and HeLa cells conditioned medium reduced the levels of two major radiation-induced genes, GADD45 and BTG2, and phosphorylation of p38. Profiling of the growth and survival factors in the conditioned medium revealed PDGF and VEGF, and IGF2, EGF, FGF-4, IGFBPs and GM-CSF to be specifically secreted from HeLa cells and CAFs, respectively. This study demonstrated radiation protective effects of CAF-cancer cell crosstalk, and identified multiple growth factors and radiation response genes that might be involved in these effects. (C) 2014 by Radiation Research Society

引用

页码：540 / 547

页数：8

共 25 条

[1]

BCL-2, in combination with MVP and IGF-1R expression, improves prediction of clinical outcome in complete response cervical carcinoma patients treated by radiochemotherapy [J].

Alberto Henriquez-Hernandez, Luis ;

Lloret, Marta ;

Pinar, Beatriz ;

Bordon, Elisa ;

Rey, Agustin ;

Lubrano, Amina ;

Carlos Lara, Pedro .

GYNECOLOGIC ONCOLOGY, 2011, 122 (03) :585-589

[2]

[Anonymous], 1989, J AM VET MED ASS, V194, P34

[3]

More than structural cells, fibroblasts create and orchestrate the tumor microenvironment [J].

Baglole, Carolyn J. ;

Ray, Denise M. ;

Bernstein, Steven H. ;

Feldon, Steven E. ;

Smith, Terry J. ;

Sime, Patricia J. ;

Phipps, Richard P. .

IMMUNOLOGICAL INVESTIGATIONS, 2006, 35 (3-4) :297-325

[4]

BERKERS JAM, 1991, J BIOL CHEM, V266, P922

[5]

Stromal fibroblasts in cancer initiation and progression [J].

Bhowmick, NA ;

Neilson, EG ;

Moses, HL .

NATURE, 2004, 432 (7015) :332-337

[6]

The binding of NCAM to FGFR1 induces a specific cellular response mediated by receptor trafficking [J].

Francavilla, Chiara ;

Cattaneo, Paola ;

Berezin, Vladimir ;

Bock, Elisabeth ;

Ami, Diletta ;

de Marco, Ario ;

Christofori, Gerhard ;

Cavallaro, Ugo .

JOURNAL OF CELL BIOLOGY, 2009, 187 (07) :1101-1116

[7]

Huang TH., 2013, Oncogene

[8]

Cancer-associated stroma fibroblasts promote pancreatic tumor progression [J].

Hwang, Rosa F. ;

Moore, Todd ;

Arumugam, Thiruvengadain ;

Ramachandran, Vijaya ;

Amos, Keith D. ;

Rivera, Armando ;

Ji, Baoan ;

Evans, Douglas B. ;

Logsdon, Craig D. .

CANCER RESEARCH, 2008, 68 (03) :918-926

[9]

Regulation of Btg2/TIS21/PC3 expression via reactive oxygen species-protein kinase C-NFκB pathway under stress conditions [J].

Imran, Muhammad ;

Lim, In Kyoung .

CELLULAR SIGNALLING, 2013, 25 (12) :2400-2412

[10]

JUNG M, 1994, CANCER RES, V54, P5194

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