Structure of Propeller-Type Parallel-Stranded RNA G-Quadruplexes, Formed by Human Telomeric RNA Sequences in K+ Solution

被引：192

作者：

Martadinata, Herry ^{[1
]}

Phan, Anh Tuan ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Phys & Math Sci, Div Phys & Appl Phys, Singapore 637371, Singapore

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 07期

关键词：

INTRAMOLECULAR G-QUADRUPLEX; G-QUARTET; CRYSTAL-STRUCTURE; DNA QUADRUPLEX; TETRAPLEX; TOPOLOGY; NMR; ARCHITECTURE; ASSIGNMENTS; INHIBITION;

D O I：

10.1021/ja806592z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Very recent studies showed that mammalian telomeres were transcribed into telomeric-repeat-containing RNAs and suggested that these RNA molecules were biologically important. Here we report on a structural study of RNA G-quadruplexes formed by human telomeric RNA sequences in K+ solution. Our data indicated that these sequences formed propeller-type parallel-stranded RNA G-quadruplexes. We have determined the NMR-based solution structure of a dimeric propeller-type RNA G-quadruplex formed by the 12-nt human telomeric RNA sequence r(UAGGGUUAGGGU). We also observed the stacking of two such propeller-type G-quadruplex blocks for the 10-nt human telomeric RNA sequence r(GGGUUAGGGU) and a higher-order G-quadruplex structure for the 9-nt human telomeric RNA sequence r(GGGUUAGGG). Based on these findings we proposed how higher-order structures might be formed by long telomeric RNA.

引用

页码：2570 / 2578

页数：9

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