Reducing product inhibition in DNA-template-controlled ligation reactions

被引：90

作者：

Dose, Christian ^{[1
]}

Ficht, Simon ^{[1
]}

Seitz, Oliver ^{[1
]}

机构：

[1] Humboldt Univ, Inst Chem, D-12489 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 32期

关键词：

FRET (fluorescence resonance energy transfer); native chemical ligation; sequence analysis; single-nucleotide polymorphism; template synthesis;

D O I：

10.1002/anie.200600464

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Loosening the zipper: A ligation-rearrangement reaction sequence is the key to signal amplification in chemical DNA-templated ligation reactions. The rearrangement increases the flexibility of the ligation intermediate, thereby reducing the affinity of the rearranged product for the DNA template. A fluorescence-resonant-energy-transfer technique is used for convenient and product-specific real-time detection of product signals. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：5369 / 5373

页数：5

相关论文

共 41 条

[21]

Hagenbuch P., 2005, Angew. Chem, V117, P6746, DOI 10.1002/ange.200501794

[22] STRUCTURE-ACTIVITY STUDIES OF THE BINDING OF MODIFIED PEPTIDE NUCLEIC-ACIDS (PNAS) TO DNA [J].

HYRUP, B ;

EGHOLM, M ;

NIELSEN, PE ;

WITTUNG, P ;

NORDEN, B ;

BUCHARDT, O .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (18) :7964-7970

[23] NONENZYMATIC TEMPLATE-DIRECTED SYNTHESIS OF INFORMATIONAL MACROMOLECULES [J].

JOYCE, GF .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1987, 52 :41-51

[24] Hybridization of DNA and PNA molecular beacons to single-stranded and double-stranded DNA targets [J].

Kuhn, H ;

Demidov, VV ;

Coull, JM ;

Fiandaca, MJ ;

Gildea, BD ;

Frank-Kamenetskii, MD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (06) :1097-1103

[25]

Li X., 2004, ANGEW CHEM, V116, P4956

[26] DNA-Templated organic synthesis: Nature's strategy for controlling chemical reactivity applied to synthetic molecules [J].

Li, XY ;

Liu, DR .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (37) :4848-4870

[27] Design of polydactyl zinc-finger proteins for unique addressing within complex genomes [J].

Liu, Q ;

Segal, DJ ;

Ghiara, JB ;

Barbas, CF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (11) :5525-5530

[28] Surface-promoted replication and exponential amplification of DNA analogues [J].

Luther, A ;

Brandsch, R ;

von Kiedrowski, G .

NATURE, 1998, 396 (6708) :245-248

[29] Nucleic acid-triggered catalytic drug release [J].

Ma, ZC ;

Taylor, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (21) :11159-11163

[30]

Mattes A, 2001, ANGEW CHEM INT EDIT, V40, P3178, DOI 10.1002/1521-3773(20010903)40:17<3178::AID-ANIE3178>3.0.CO

← 1 2 3 4 5 →