Characterization of human CD25+ CD4+ T cells in thymus, cord and adult blood

被引:180
作者
Wing, K [1 ]
Ekmark, A [1 ]
Karlsson, H [1 ]
Rudin, A [1 ]
Suri-Payer, E [1 ]
机构
[1] Univ Gothenburg, Dept Rheumatol, S-41346 Gothenburg, Sweden
关键词
D O I
10.1046/j.1365-2567.2002.01412.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CD4(+) CD25(+) regulatory T cells prevent organ-specific autoimmune diseases in various animal models. We analysed human lymphoid tissues to identify similar CD25(+) regulatory T cells. Adult peripheral blood contained two populations of CD4(+) T cells that expressed CD25 at different densities. The larger population (approximate to40%) expressed intermediate levels of CD25 (CD25(+) ) and displayed a memory T-cell phenotype (CD45RA(-) /RO+ , CD45RB(low) , CD95(+) , CD62L(low) , CD38(low) ). The smaller population of cells (approximate to2%) expressed very high levels of CD25 (CD25(++) ). In addition to the activation/memory T-cell antigens mentioned above they also expressed intracellular CD152 (CTLA-4) as well as enhanced levels of cell-surface CD122, similar to the murine CD4(+) CD25(+) regulatory counterpart. To exclude that the CD25(++) cells had not been recently primed by external antigen we analysed cord blood and thymus. CD25(++) , CD152(+) and CD122(++) cells were present in paediatric thymus (10% of CD4(+) CD8(-) thymocytes) expressing signs of recent selection (CD69(+) ) and in cord blood (5% of CD4(+) cells) where they showed a naive phenotype. In addition, cord blood contained a small population of CD25(+) cells (approximate to2% of CD4 T cells) that were CD152(-) and CD122(low) and displayed signs of activation. Together with published data that CD25(+) CD25(++) cells from the thymus and peripheral blood are regulatory, our results suggest that regulatory CD25(+) T cells leave the thymus in a naive state and become activated in the periphery.
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收藏
页码:190 / 199
页数:10
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