Superheated heavy water as the eluent for HPLC-NMR and HPLC-NMR-MS of model drugs

被引：57

作者：

Smith, RM ^{[1
]}

Chienthavorn, O

Wilson, ID

Wright, B

Taylor, SD

机构：

[1] Loughborough Univ Technol, Dept Chem, Loughborough LE11 3TU, Leics, England

[2] Zeneca Pharmaceut, Macclesfield SK10 4TG, Cheshire, England

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 20期

关键词：

D O I：

10.1021/ac9905470

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Superheated heavy water can be used as the eluent for reversed-phase chromatography with on-line HPLC-NMR and HPLC-NMR-MS detection. The method has been demonstrated for the separation of model drugs (analgesics and caffeine) on a poly(styrene - divinylbenzene) stationary phase in isothermal and temperature-programmed modes. One- and two-dimensional spectra could be obtained with less interference from the mobile phase than with conventional HPLC-NMR eluents. Unlike supercritical fluid chromatography-NMR, the spectra could be measured at room temperature and atmospheric pressure. The combination of superheated water HPLC-NMR-MS chromatography enables both NMR and MS spectra to be obtained simultaneously for the same sample.

引用

页码：4493 / 4497

页数：5

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