The application of high performance liquid chromatography, coupled to nuclear magnetic resonance spectroscopy and mass spectrometry (HPLC-NMR-MS), to the characterisation of ibuprofen metabolites from human urine

被引:55
作者
Clayton, E [1 ]
Taylor, S [1 ]
Wright, B [1 ]
Wilson, ID [1 ]
机构
[1] Zeneca Pharmaceut, Macclesfield SK10 4TG, Cheshire, England
关键词
column liquid chromatography; NMR and MS detection; ibuprofen metabolites; urine extracts;
D O I
10.1007/BF02466530
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The use of MPLC-NMR-MS for the detection and identification of the metabolites elf ibuprofen present in a solid phase extract of human urine is described, Gradient reversed-phase HPLC was used to separate the components present in the extract, which were then characterised by a combination of stopped-flow H-1 NMR and on line electrospray-MS, This approach led to the rapid identification of the known phase 1 human metabolites of ibuprofen, including hydroxy- and carboxy-metabolites, together with their respective glucuronide conjugates. In addition a probable artefact resulting from the dehydration of one of the side chain-hydroxylated glucuronides was also identified.
引用
收藏
页码:264 / 270
页数:7
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