NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA

被引：138

作者：

Jiang, Shan ^{[1
]}

Zhang, Yong ^{[1
]}

Lim, Kian Meng ^{[2
]}

Sim, Eugene K. W. ^{[3
]}

Ye, Lei ^{[4
]}

机构：

[1] Natl Univ Singapore, Fac Engn, Div Bioengn, Singapore 117576, Singapore

[2] Natl Univ Singapore, Dept Mech Engn, Singapore 119260, Singapore

[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg, Singapore 117597, Singapore

[4] Natl Univ Singapore, Natl Univ Med Inst, Singapore 117597, Singapore

来源：

NANOTECHNOLOGY | 2009年 / 20卷 / 15期

关键词：

CONVERTING PHOSPHOR REPORTERS; MODIFIED SILICA-NANOPARTICLES; IN-VIVO; RNA INTERFERENCE; HER2/NEU GENE; LUMINESCENCE; EXPRESSION; PROBES; CELLS;

D O I：

10.1088/0957-4484/20/15/155101

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Near-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF4 upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules.

引用

页数：9

共 32 条

[1]

Bharali DJ, 2005, P NATL ACAD SCI USA, V102, P11539, DOI 10.1073/pnas.0504926102

[2] Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals [J].

Chatteriee, Dev K. ;

Rufalhah, Abdul J. ;

Zhang, Yong .

BIOMATERIALS, 2008, 29 (07) :937-943

[3] Quantum dots to monitor RNAi delivery and improve gene silencing [J].

Chen, AA ;

Derfus, AM ;

Khetani, SR ;

Bhatia, SN .

NUCLEIC ACIDS RESEARCH, 2005, 33 (22) :e190

[4] Sleeping Beauty-mediated down-regulation of huntingtin expression by RNA interference [J].

Chen, ZYJ ;

Kren, BT ;

Wong, PYP ;

Low, WC ;

Steer, CJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 329 (02) :646-652

[5]

Corstjens P, 2001, CLIN CHEM, V47, P1885

[6] Nanoprobes with near-infrared persistent luminescence for in vivo imaging [J].

de Chermont, Quentin le Masne ;

Chaneac, Corinne ;

Seguin, Johanne ;

Pelle, Fabienne ;

Maitrejean, Serge ;

Jolivet, Jean-Pierre ;

Gourier, Didier ;

Bessodes, Michel ;

Scherman, Daniel .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (22) :9266-9271

[7] Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods [J].

Durr, Nicholas J. ;

Larson, Timothy ;

Smith, Danielle K. ;

Korgel, Brian A. ;

Sokolov, Konstantin ;

Ben-Yakar, Adela .

NANO LETTERS, 2007, 7 (04) :941-945

[8] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[9] Silencing of the HER2/neu gene by siRNA inhibits proliferation and induces apoptosis in HER2/neu-overexpressing breast cancer cells [J].

Faltus, T ;

Yuan, JP ;

Zimmer, B ;

Krämer, A ;

Loibl, S ;

Kaufmann, M ;

Strebhardt, K .

NEOPLASIA, 2004, 6 (06) :786-795

[10] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

← 1 2 3 4 →