NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA

被引：138

作者：

Jiang, Shan ^{[1
]}

Zhang, Yong ^{[1
]}

Lim, Kian Meng ^{[2
]}

Sim, Eugene K. W. ^{[3
]}

Ye, Lei ^{[4
]}

机构：

[1] Natl Univ Singapore, Fac Engn, Div Bioengn, Singapore 117576, Singapore

[2] Natl Univ Singapore, Dept Mech Engn, Singapore 119260, Singapore

[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg, Singapore 117597, Singapore

[4] Natl Univ Singapore, Natl Univ Med Inst, Singapore 117597, Singapore

来源：

NANOTECHNOLOGY | 2009年 / 20卷 / 15期

关键词：

CONVERTING PHOSPHOR REPORTERS; MODIFIED SILICA-NANOPARTICLES; IN-VIVO; RNA INTERFERENCE; HER2/NEU GENE; LUMINESCENCE; EXPRESSION; PROBES; CELLS;

D O I：

10.1088/0957-4484/20/15/155101

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Near-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF4 upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules.

引用

页数：9

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