Expression of Cry1Ac cadherin receptors in insect midgut and cell lines

被引：39

作者：

Aimanova, Karlygash G. ^{[1
]}

Zhuang, Meibao ^{[1
]}

Gill, Sarjeet S. ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Cell Biol & Neurosci, Riverside, CA 92521 USA

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2006年 / 92卷 / 03期

关键词：

cadherin-like protein; Heliothis virescens; immunolocalization; Bacillus thuringiensis; cell expression;

D O I：

10.1016/j.jip.2006.04.011

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Cadherin-like proteins have been identified as putative receptors for the Bacillus thuringiensis Cry1A proteins in Heliothis virescens and Manduca sexta. Immunohistochemistry showed the cadherin-like proteins are present in the insect midgut apical membrane, which is the target site of Cry toxins. This subcellular localization is distinct from that of classical cadherins, which are usually present in cell-cell junctions. Immuno reactivity of the cadherin-like protein in the insect midgut was enhanced by Cry1Ac ingestion. We also generated a stable cell line Flp-InT-REX-293/Full-CAD (CAD/293) that expressed the H. virescens cadherin. As expected, the cadherin-like protein was mainly localized in the cell membrane. Interestingly, toxin treatment of CAD/293 cells caused this protein to relocalize to cell membrane subdomains. In addition, expression of H. virescens cadherin-like protein affects cell-cell contact and cell membrane integrity when the cells are exposed to activated Cry1Ab/Cry1Ac. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：178 / 187

页数：10

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