pH measurement as quality control on human post mortem brain tissue: a study of the BrainNet Europe consortium

被引：92

作者：

Monoranu, C. M. ^{[1
]}

Apfelbacher, M. ^{[1
,2
]}

Gruenblatt, E. ^{[2
]}

Puppe, B. ^{[1
]}

Alafuzoff, I. ^{[6
]}

Ferrer, I. ^{[7
]}

Al-Saraj, S. ^{[8
]}

Keyvani, K. ^{[3
]}

Schmitt, A. ^{[4
]}

Falkai, P. ^{[4
]}

Schittenhelm, J. ^{[5
]}

Halliday, G. ^{[9
,10
]}

Kril, J. ^{[11
]}

Harper, C. ^{[11
]}

McLean, C. ^{[12
]}

Riederer, P. ^{[2
]}

Roggendorf, W. ^{[1
]}

机构：

[1] Univ Wurzburg, Inst Pathol, Dept Neuropathol, D-97080 Wurzburg, Germany

[2] Univ Wurzburg, Natl Parkinson Fdn Ctr, Excellence Res Lab, Clin & Policlin Psychiat Psychosomat & Psychother, D-97080 Wurzburg, Germany

[3] Univ Hosp, Inst Neuropathol, Munster, Germany

[4] Univ Gottingen, Clin Psychiat & Psychotherapy, Gottingen, Germany

[5] Univ Tubingen, Inst Brain Res Neuropathol, Tubingen, Germany

[6] Univ Kuopio, Dept Clin Med, FIN-70211 Kuopio, Finland

[7] Univ Barcelona, Inst Neuropatol, Barcelona, Spain

[8] London Inst Psychiat, Dept Clin Neuropathol, London, England

[9] Prince Wales Med Res Inst, Sydney, NSW, Australia

[10] Univ New S Wales, Sydney, NSW, Australia

[11] Univ Sydney, Dept Pathol, Sydney, NSW 2006, Australia

[12] Monash Univ, Dept Anat Pathol, Alfred Hosp, Prahran, Vic, Australia

来源：

NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY | 2009年 / 35卷 / 03期

关键词：

agonal state; brain banking; brain ischaemia; human post mortem brain tissue; pH; AGONAL STATUS; GENE-EXPRESSION; RNA; DISSOCIATION; PREMORTEM; LACTATE;

D O I：

10.1111/j.1365-2990.2008.01003a.x

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Aims: Most brain diseases are complex entities. Although animal models or cell culture experiments mimic some disease aspects, human post mortem brain tissue remains essential to advance our understanding of brain diseases using biochemical and molecular techniques. Post mortem artefacts must be properly understood, standardized, and either eliminated or factored into such experiments. Here we examine the influence of several premortem and post mortem factors on pH, and discuss the role of pH as a biochemical marker for brain tissue quality. Methods: We assessed brain tissue pH in 339 samples from 116 brains provided by 8 different European and 2 Australian brain bank centres. We correlated brain pH with tissue source, post mortem delay, age, gender, freezing method, storage duration, agonal state and brain ischaemia. Results: Our results revealed that only prolonged agonal state and ischaemic brain damage influenced brain tissue pH next to repeated freeze/thaw cycles. Conclusions: pH measurement in brain tissue is a good indicator of premortem events in brain tissue and it signals limitations for post mortem investigations.

引用

页码：329 / 337

页数：9

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