Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR

被引：20

作者：

Johnson, JR

Owens, K

Sabate, M

Prats, G

机构：

[1] Minneapolis VA Med Ctr, Mucosal & Vaccinre Res Ctr, Minneapolis, MN 55417 USA

[2] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA

[3] Val Hebron Hosp, Clin Microbiol Lab, Barcelona, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 08期

关键词：

D O I：

10.1128/JCM.42.8.3841-3843.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Primers specific for Escherichia coli 015:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the 015:K52:111 clonal group, including 162 diverse clinical and reference E. coli isolates.

引用

收藏

页码：3841 / 3843

页数：3

相关论文

共 24 条

[1]

Berg Douglas E., 1994, Methods in Molecular and Cellular Biology, V5, P13

[2] Variability in presence-absence scoring of AP PCR fingerprints affects computer matching of bacterial isolates [J].

Burr, MD ;

Pepper, IL .

JOURNAL OF MICROBIOLOGICAL METHODS, 1997, 29 (01) :63-68

[3]

ELLSWORTH DL, 1993, BIOTECHNIQUES, V14, P214

[4] PHYLOGENETIC DISTRIBUTION OF BRANCHED RNA-LINKED MULTICOPY SINGLE-STRANDED-DNA AMONG NATURAL ISOLATES OF ESCHERICHIA-COLI [J].

HERZER, PJ ;

INOUYE, S ;

INOUYE, M ;

WHITTAM, TS .

JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6175-6181

[5] Rapid and specific detection of Escherichia coli clonal group a by gene-specific PCR [J].

Johnson, JR ;

Owens, K ;

Manges, AR ;

Riley, LW .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (06) :2618-2622

[6] Improved repetitive-element PCR fingerprinting for resolving pathogenic and nonpathogenic phylogenetic groups within Escherichia coli [J].

Johnson, JR ;

O'Bryan, TT .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2000, 7 (02) :265-273

[7] Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and/or extended-spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans [J].

Johnson, JR ;

Kuskowski, MA ;

Owens, K ;

Gajewski, A ;

Winokur, PL .

JOURNAL OF INFECTIOUS DISEASES, 2003, 188 (05) :759-768

[8] A disseminated multidrug-resistant clonal group of uropathogenic Escherichia coli in pyelonephritis [J].

Johnson, JR ;

Manges, AR ;

O'Bryan, TT ;

Riley, LW .

LANCET, 2002, 359 (9325) :2249-2251

[9] Global molecular epidemiology of the O15:K52:H1 extraintestinal pathogenic Escherichia coli clonal group:: Evidence of distribution beyond Europe [J].

Johnson, JR ;

Stell, AL ;

O'Bryan, TT ;

Kuskowski, M ;

Nowicki, B ;

Johnson, C ;

Maslow, JN ;

Kaul, A ;

Kavle, J ;

Prats, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) :1913-1923

[10] Ongoing horizontal and vertical transmission of virulence genes and papA alleles among Escherichia coli blood isolates from patients with diverse-source bacteremia [J].

Johnson, JR ;

O'Bryan, TT ;

Kuskowski, M ;

Maslow, JN .

INFECTION AND IMMUNITY, 2001, 69 (09) :5363-5374