Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR

被引：20

作者：

Johnson, JR

Owens, K

Sabate, M

Prats, G

机构：

[1] Minneapolis VA Med Ctr, Mucosal & Vaccinre Res Ctr, Minneapolis, MN 55417 USA

[2] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA

[3] Val Hebron Hosp, Clin Microbiol Lab, Barcelona, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 08期

关键词：

D O I：

10.1128/JCM.42.8.3841-3843.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Primers specific for Escherichia coli 015:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the 015:K52:111 clonal group, including 162 diverse clinical and reference E. coli isolates.

引用

收藏

页码：3841 / 3843

页数：3

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