Peripheral levels of caspase-1 mRNA correlate with disease activity in patients with multiple sclerosis; a preliminary study

被引:57
作者
Furlan, R
Filippi, M
Bergami, A
Rocca, MA
Martinelli, V
Poliani, PL
Grimaldi, LME
Desina, G
Comi, G
Martino, G
机构
[1] San Raffaele Sci Inst, DIBIT, Expt Neuroimmunotherapy Unit, I-20132 Milan, Italy
[2] San Raffaele Sci Inst, Neuroimaging Res Unit, I-20132 Milan, Italy
[3] San Raffaele Sci Inst, Clin Trials Unit, I-20132 Milan, Italy
[4] San Raffaele Sci Inst, Dept Neurosci, Neuroimmunol Unit, I-20132 Milan, Italy
关键词
multiple sclerosis; caspase-1; proinflammatory cytokines;
D O I
10.1136/jnnp.67.6.785
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
The cysteine protease caspase-1 plays a crucial part in the inflammatory process due to its ability to proteolitically activate proinflammatory cytokine precursors, such as interleukin (IL)-1 beta and IL-18. Multiple sclerosis is a chronic inflammatory demyelinating disease of the CNS in which the pathogenic process is mainly orchestrated by proinflammatory cytokines. The role of caspase-1 in multiple sclerosis was evaluated by measuring its mRNA levels in peripheral blood mononuclear cells (PBMCs) from seven patients with relapsing-remitting multiple sclerosis every 15 days over a 1 year period. The recorded levels were compared with clinical and MRI evidence of disease activity. Brain MRI was performed monthly in each patient. Caspase-1 mRNA levels were significantly increased in PBMCs from patients with multiple sclerosis compared with healthy controls (p < 0.001). In patients with multiple sclerosis, a twofold to threefold increase of caspase-1 mRNA mean level was found in the week preceding an acute attack (p < 0.05). The magnitude of caspase-1 mRNA increase correlated with the number of new (p = 0.01) but not persisting gadolinium enhancing brain MRT lesions. In conclusion, caspase-1 might be involved in the immune mediated process underlying CNS inflammation and might represent a suitable peripheral immunological marker of disease activity in multiple sclerosis.
引用
收藏
页码:785 / 788
页数:4
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