High-level expression of a recombinant thermostable phytase in Bacillus subtilis

被引：24

作者：

Kim, YO ^{[1
]}

Lee, JK ^{[1
]}

Oh, BC ^{[1
]}

Oh, TK ^{[1
]}

机构：

[1] Microbial & Enzyme RU, KRIBB, Taejon 305600, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 12期

关键词：

Bacillus subtilis; phytase; phytate; overexpression;

D O I：

10.1271/bbb.63.2205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An efficient expression system was developed in Bacillus subtilis for the large scale production of phytase. The phytase gene with a native promoter derived from Bacillus amyloliquefaciens was cloned in the Bacillus expression vector pJH27 under a strong BJ27 promoter and its expression was optimized. The expression of the phytase gene occurred during late exponential growth and the extracellular phytase production was 2.0 units/ml, which constituted over 90% of the total protein. The yield was 100-fold higher than wild type Bacillus amyloliquefaciens DS11.

引用

页码：2205 / 2207

页数：3

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