A Role for Barley CRYPTOCHROME1 in Light Regulation of Grain Dormancy and Germination

被引:120
作者
Barrero, Jose M. [1 ]
Downie, A. Bruce [2 ]
Xu, Qian [1 ]
Gubler, Frank [1 ]
机构
[1] CSIRO, Plant Ind, Canberra, ACT 2601, Australia
[2] Univ Kentucky, Dept Hort, Lexington, KY 40546 USA
基金
美国食品与农业研究所;
关键词
BLUE-LIGHT; ABSCISIC-ACID; GENE-EXPRESSION; GREEN LIGHT; ARABIDOPSIS; PHOTOTROPIN; PHOTORECEPTORS; PHYTOCHROME; PROTEIN; PHOTOMORPHOGENESIS;
D O I
10.1105/tpc.113.121830
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is well known that abscisic acid (ABA) plays a central role in the regulation of seed dormancy and that transcriptional regulation of genes encoding ABA biosynthetic and degradation enzymes is responsible for determining ABA content. However, little is known about the upstream signaling pathways impinging on transcription to ultimately regulate ABA content or how environmental signals (e. g., light and cold) might direct such expression in grains. Our previous studies indicated that light is a key environmental signal inhibiting germination in dormant grains of barley (Hordeum vulgare), wheat (Triticum aestivum), and Brachypodium distachyon and that this effect attenuates as after-ripening progresses further. We found that the blue component of the light spectrum inhibits completion of germination in barley by inducing the expression of the ABA biosynthetic gene 9-cis-epoxycarotenoid dioxygenase and dampening expression of ABA 8'-hydroxylase, thus increasing ABA content in the grain. We have now created barley transgenic lines downregulating the genes encoding the blue light receptors CRYTOCHROME (CRY1) and CRY2. Our results demonstrate that CRY1 is the key receptor perceiving and transducing the blue light signal in dormant grains.
引用
收藏
页码:1094 / 1104
页数:11
相关论文
共 59 条
[51]   HvVRN2 responds to daylength, whereas HvVRN1 is regulated by vernalization and developmental status [J].
Trevaskis, B ;
Hemming, MN ;
Peacock, WJ ;
Dennis, ES .
PLANT PHYSIOLOGY, 2006, 140 (04) :1397-1405
[52]   Improved vectors for Agrobacterium tumefaciens-mediated transformation of monocot plants [J].
Wang, MB ;
Li, ZY ;
Matthews, PR ;
Upadhyaya, NM .
INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF TROPICAL AND SUBTROPICAL SPECIES - PART II, 1998, (461) :401-407
[53]   CONTRIBUTIONS OF GREEN LIGHT TO PLANT GROWTH AND DEVELOPMENT [J].
Wang, Yihai ;
Folta, Kevin M. .
AMERICAN JOURNAL OF BOTANY, 2013, 100 (01) :70-78
[54]   Composition and phylogenetic analysis of wheat cryptochrome gene family [J].
Xu, Pei ;
Zhu, Hui Lan ;
Xu, Hai Bin ;
Zhang, Zheng Zhi ;
Zhang, Cai Qin ;
Zhang, Li Xia ;
Ma, Zheng Qiang .
MOLECULAR BIOLOGY REPORTS, 2010, 37 (02) :825-832
[55]   Wheat Cryptochromes: Subcellular Localization and Involvement in Photomorphogenesis and Osmotic Stress Responses [J].
Xu, Pei ;
Xiang, Yang ;
Zhu, Huilan ;
Xu, Haibin ;
Zhang, Zhengzhi ;
Zhang, Caiqin ;
Zhang, Lixia ;
Ma, Zhengqiang .
PLANT PHYSIOLOGY, 2009, 149 (02) :760-774
[56]  
Yu X., 2010, THE ARABIDOPSIS BOOK, V8, pe0135, DOI 10.1199/tab.0135
[57]   Two early light-indudible protein (ELIP) cDNAs from the resurrection plant Tortula ruralis are differentially expressed in response to desiccation, rehydration, salinity, and high light [J].
Zeng, O ;
Chen, XB ;
Wood, AJ .
JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (371) :1197-1205
[58]   Green Light Induces Shade Avoidance Symptoms [J].
Zhang, Tingting ;
Maruhnich, Stefanie A. ;
Folta, Kevin M. .
PLANT PHYSIOLOGY, 2011, 157 (03) :1528-1536
[59]   Functional and signaling mechanism analysis of rice CRYPTOCHROME 1 [J].
Zhang, Yan-Chun ;
Gong, Song-Fu ;
Li, Qing-Hua ;
Sang, Yi ;
Yang, Hong-Quan .
PLANT JOURNAL, 2006, 46 (06) :971-983