Partial purification and characterization of sucrose phosphate synthase from preclimacteric and climacteric bananas

被引：14

作者：

Nascimento, JRO ^{[1
]}

Cordenunsi, BR ^{[1
]}

Lajolo, FM ^{[1
]}

机构：

[1] UNIV SAO PAULO,FCF,DEPT ALIMENTOS & NUTR EXPT,BR-05389970 SAO PAULO,BRAZIL

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1997年 / 45卷 / 04期

关键词：

Musa acuminata; banana; sucrose phosphate synthase; ripening; sucrose;

D O I：

10.1021/jf960407g

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Sucrose phosphate synthase (SPS) (EC 2.4.1.14) was purified from bananas (Musa acuminata cv. Nanicao) at different developmental and ripening stages (70, 110, and 130 days after anthesis), corresponding to tissue with different structure and composition. Banana SPS exists in three polymeric forms as observed by electrophoresis and immunoblotting, similar in all stages studied, with M-W respectively of 180, 254, and 686 kDa. The three forms have the same 116 kDa subunit, and their activity increased during ripening associated with increased protein synthesis as shown by Western blotting.

引用

页码：1103 / 1107

页数：5

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