Integrative analyses reveal transcriptome-proteome correlation in biological pathways and secondary metabolism clusters in A. flavus in response to temperature

被引：79

作者：

Bai, Youhuang

Wang, Sen

Zhong, Hong

Yang, Qi

Zhang, Feng

Zhuang, Zhenhong

Yuan, Jun

Nie, Xinyi

Wang, Shihua ^{[1
]}

机构：

[1] Fujian Agr & Forestry Univ, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Fuzhou 350002, Peoples R China

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

中国国家自然科学基金;

关键词：

ASPERGILLUS-FLAVUS; ENVIRONMENTAL-FACTORS; GENE-TRANSCRIPTION; WATER ACTIVITY; EXPRESSION; BIOSYNTHESIS; GROWTH; QUANTIFICATION; IDENTIFICATION; CARBONARIUS;

D O I：

10.1038/srep14582

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To investigate the changes in transcript and relative protein levels in response to temperature, complementary transcriptomic and proteomic analyses were used to identify changes in Aspergillus flavus grown at 28 degrees C and 37 degrees C. A total of 3,886 proteins were identified, and 2,832 proteins were reliably quantified. A subset of 664 proteins was differentially expressed upon temperature changes and enriched in several Kyoto Encyclopedia of Genes and Genomes pathways: translation-related pathways, metabolic pathways, and biosynthesis of secondary metabolites. The changes in protein profiles showed low congruency with alterations in corresponding transcript levels, indicating that post-transcriptional processes play a critical role in regulating the protein level in A. flavus. The expression pattern of proteins and transcripts related to aflatoxin biosynthesis showed that most genes were up-regulated at both the protein and transcript level at 28 degrees C. Our data provide comprehensive quantitative proteome data of A. flavus at conducive and nonconducive temperatures.

引用

页数：13

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