Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria

被引:149
作者
Nishiwaki, T
Iwasaki, H
Ishiura, M
Kondo, T
机构
[1] Nagoya Univ, Ctr Gene Res, Chikusa Ku, Grad Sch Sci,Div Biol Sci, Nagoya, Aichi 4648602, Japan
[2] Nagoya Univ, Ctr Gene Res, Chikusa Ku, Grad Sch Sci,Div Mat Sci, Nagoya, Aichi 4648602, Japan
关键词
D O I
10.1073/pnas.97.1.495
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A negative feedback control of kaiC expression by KaiC protein has been proposed to generate a basic oscillation of the circadian clock in the cyanobacterium Synechococcus sp, PCC 7942, KaiC has two P loops or Walker's motif As, that are potential ATP-/GTP-binding motifs and DXXG motifs conserved in various GTP-binding proteins. Herein, we demonstrate that in vitro KaiC binds ATP and, with lower affinity, GTP, Point mutation by site-directed mutagenesis of P loop 1 completely nullified the circadian rhythm of kaiBC expression and markedly reduced ATP-binding activity. Moreover, KaiC can be autophosphorylated in vitro. These results suggest that the nucleotide-binding activity of KaiC plays important roles in the generation of circadian oscillation in cyanobacteria.
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页码:495 / 499
页数:5
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