Renewable phenol biosensor based on a tyrosinase-colloidal gold modified carbon paste electrode

被引:139
作者
Liu, SQ [1 ]
Yu, JH [1 ]
Ju, HX [1 ]
机构
[1] Nanjing Univ, Inst Biol Chem, Dept Chem, State Key Lab Coordinat Chem, Nanjing 210093, Peoples R China
来源
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2003年 / 540卷
基金
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词
tyrosinase; biosensor; phenol; carbon paste electrode; colloidal gold;
D O I
10.1016/S0022-0728(02)01276-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel renewable tyrosinase-based biosensor was developed for the detection of phenol by immobilizing tyrosinase on a colloidal gold modified carbon paste electrode. Anionic colloidal gold was beneficial to the immobilization of tyrosinase and to the retention of its bioactivity to a large extent. The biosensor showed a sensitive electrochemical response to the reduction of the oxidation product of phenol by dissolved O-2 in the presence of immobilized tyrosinase. The effects of pH, operating potential and the volume of the colloidal gold solution for sensor preparation on the amperometric response were explored for optimum analytical performance. The best performing biosensor exhibited a fast response (less than 5 s), a high sensitivity (12.3 muA cm(-2) muM(-1)) and good storage stability for monitoring phenol. The linear range spanned the concentration of phenol from 4 to 48 muM with a correlation coefficient of 0.9973 (n = 12) and a detection limit of 6.1 nM at 3sigma. The response showed Michaelis-Menten behavior at larger phenol concentrations. The K-M(app) value of immobilized tyrosinase on colloidal gold was calculated to be (53.6 +/- 3.2) AM using phenol as the substrate. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:61 / 67
页数:7
相关论文
共 37 条
  • [1] Zeptomole-detecting biosensor for alkaline phosphatase in an electrochemical immunoassay for 2,4-dichlorophenoxyacetic acid
    Bauer, CG
    Eremenko, AV
    EhrentreichForster, E
    Bier, FF
    Makower, A
    Halsall, HB
    Heineman, WR
    Scheller, FW
    [J]. ANALYTICAL CHEMISTRY, 1996, 68 (15) : 2453 - 2458
  • [2] SEPARATION OF PHENOLS BY PACKED-COLUMN SUPERCRITICAL FLUID CHROMATOGRAPHY
    BERGER, TA
    DEYE, JF
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1991, 29 (02) : 54 - 59
  • [3] BESCOMBES JL, 1995, ANAL CHIM ACTA, V317, P275
  • [4] SEPARATION OF PHENOLS AND AROMATIC-HYDROCARBONS FROM BIOMASS TAR USING AMINOPROPYLSILANE NORMAL-PHASE LIQUID-CHROMATOGRAPHY
    BRAGE, C
    SJOSTROM, K
    [J]. JOURNAL OF CHROMATOGRAPHY, 1991, 538 (02): : 303 - 310
  • [5] Morphology-dependent electrochemistry of cytochrome c at Au colloid-modified SnO2 electrodes
    Brown, KR
    Fox, AP
    Natan, MJ
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (05) : 1154 - 1157
  • [6] Persistence of selected pesticides and their phenolic transformation products in natural waters using off-line liquid solid extraction followed by liquid chromatographic techniques
    Castillo, M
    Domingues, R
    Alpendurada, MF
    Barcelo, D
    [J]. ANALYTICA CHIMICA ACTA, 1997, 353 (01) : 133 - 142
  • [7] A comparison of amperometric screen-printed, carbon electrodes and their application to the analysis of phenolic compounds present in beers
    Cummings, EA
    Linquette-Mailley, S
    Mailley, P
    Cosnier, S
    Eggins, BR
    McAdams, ET
    [J]. TALANTA, 2001, 55 (05) : 1015 - 1027
  • [8] ORGANIZATION OF AU COLLOIDS AS MONOLAYER FILMS ONTO ITO GLASS SURFACES - APPLICATION OF THE METAL COLLOID FILMS AS BASE INTERFACES TO CONSTRUCT REDOX-ACTIVE MONOLAYERS
    DORON, A
    KATZ, E
    WILLNER, I
    [J]. LANGMUIR, 1995, 11 (04) : 1313 - 1317
  • [9] Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment:: a review
    Durán, N
    Esposito, E
    [J]. APPLIED CATALYSIS B-ENVIRONMENTAL, 2000, 28 (02) : 83 - 99
  • [10] Michaelis constants of mushroom tyrosinase with respect to oxygen in the presence of monophenols and diphenols
    Fenoll, LG
    Rodríguez-López, JN
    García-Molina, F
    García-Cánovas, F
    Tudela, J
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2002, 34 (04) : 332 - 336