Promoter and Riboswitch Control of the Mg2+ Transporter MgtA from Salmonella enterica

被引：38

作者：

Cromie, Michael J. ^{[1
]}

Groisman, Eduardo A. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Mol Microbiol, Howard Hughes Med Inst, St Louis, MO 63110 USA

来源：

JOURNAL OF BACTERIOLOGY | 2010年 / 192卷 / 02期

关键词：

ENVELOPE STRESS-RESPONSE; BACTERIAL SENSOR KINASE; ESCHERICHIA-COLI; MAGNESIUM TRANSPORT; CHROMOSOMAL GENES; TYPHIMURIUM; TRANSCRIPTION; HOMEOSTASIS; EXPRESSION; VIRULENCE;

D O I：

10.1128/JB.01239-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The MgtA protein from Salmonella enterica serovar Typhimurium mediates Mg2+ uptake from the periplasm into the cytoplasm. Here we report that the PhoP/PhoQ two-component regulatory system, which responds to periplasmic Mg2+, governs mgtA transcription initiation at all investigated Mg2+ concentrations and that the Mg2+-sensing 5' leader region of the mgtA gene controls transcription elongation into the mgtA coding region when Salmonella is grown in media with < 50 mu M Mg2+. Overexpression of the Mg2+ transporter CorA, which is believed to increase cytoplasmic Mg2+ levels, decreased mgtA transcription in a manner dependent on a functional mgtA 5' leader.

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收藏

页码：604 / 607

页数：4

相关论文

共 24 条

[1] Recognition of antimicrobial peptides by a bacterial sensor kinase [J].

Bader, MW ;

Sanowar, S ;

Daley, ME ;

Schneider, AR ;

Cho, US ;

Xu, WQ ;

Klevit, RE ;

Le Moual, H ;

Miller, S .

CELL, 2005, 122 (03) :461-472

[2] mgtA expression is induced by rob overexpression and mediates a Salmonella enterica resistance phenotype [J].

Barchiesi, Julieta ;

Castelli, Maria E. ;

Soncini, Fernando C. ;

Vescovi, Eleonora Garcia .

JOURNAL OF BACTERIOLOGY, 2008, 190 (14) :4951-4958

[3] Mg2+ homeostasis and avoidance of metal toxicity [J].

Chamnongpol, S ;

Groisman, EA .

MOLECULAR MICROBIOLOGY, 2002, 44 (02) :561-571

[4] Activated by Different Signals, the PhoP/PhoQ Two-Component System Differentially Regulates Metal Uptake [J].

Choi, Eunna ;

Groisman, Eduardo A. ;

Shin, Dongwoo .

JOURNAL OF BACTERIOLOGY, 2009, 191 (23) :7174-7181

[5] An RNA sensor for intracellular Mg2+ [J].

Cromie, MJ ;

Shi, YX ;

Latifi, T ;

Groisman, EA .

CELL, 2006, 125 (01) :71-84

[6] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].

Datsenko, KA ;

Wanner, BL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645

[7] Loss of Hfq activates the σE-dependent envelope stress response in Salmonella enterica [J].

Figueroa-Bossi, Nara ;

Lemire, Sebastien ;

Maloriol, Daniela ;

Balbontin, Roberto ;

Casadesus, Josep ;

Bossi, Lionello .

MOLECULAR MICROBIOLOGY, 2006, 62 (03) :838-852

[8] The pleiotropic two-component regulatory system PhoP-PhoQ [J].

Groisman, EA .

JOURNAL OF BACTERIOLOGY, 2001, 183 (06) :1835-1842

[9] Hfq modulates the σE-mediated envelope stress response and the σ32-mediated cytoplasmic stress response in Escherichia coli [J].

Guisbert, Eric ;

Rhodius, Virgil A. ;

Ahuja, Nidhi ;

Witkin, Emily ;

Gross, Carol A. .

JOURNAL OF BACTERIOLOGY, 2007, 189 (05) :1963-1973

[10] MAGNESIUM TRANSPORT IN SALMONELLA-TYPHIMURIUM - GENETIC-CHARACTERIZATION AND CLONING OF 3 MAGNESIUM TRANSPORT LOCI [J].

HMIEL, SP ;

SNAVELY, MD ;

FLORER, JB ;

MAGUIRE, ME ;

MILLER, CG .

JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :4742-4751