Synthetic Gene Networks That Count

被引：445

作者：

Friedland, Ari E. ^{[1
,2
]}

Lu, Timothy K. ^{[1
,2
,3
]}

Wang, Xiao ^{[1
,2
]}

Shi, David ^{[1
,2
]}

Church, George ^{[3
,4
]}

Collins, James J. ^{[1
,2
]}

机构：

[1] Boston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr BioDynam, Boston, MA 02215 USA

[2] Boston Univ, Ctr Adv Biotechnol, Boston, MA 02215 USA

[3] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[4] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

来源：

SCIENCE | 2009年 / 324卷 / 5931期

关键词：

MAMMALIAN-CELLS; DIRECTED EVOLUTION; ESCHERICHIA-COLI; EXPRESSION; SWITCH; RIBOREGULATORS; BACTERIOPHAGE; RECOMBINASE; OSCILLATOR; PATTERN;

D O I：

10.1126/science.1172005

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Synthetic gene networks can be constructed to emulate digital circuits and devices, giving one the ability to program and design cells with some of the principles of modern computing, such as counting. A cellular counter would enable complex synthetic programming and a variety of biotechnology applications. Here, we report two complementary synthetic genetic counters in Escherichia coli that can count up to three induction events: the first, a riboregulated transcriptional cascade, and the second, a recombinase-based cascade of memory units. These modular devices permit counting of varied user-defined inputs over a range of frequencies and can be expanded to count higher numbers.

引用

页码：1199 / 1202

页数：4

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