Light-induced conformational changes and energy transfer in red fluorescent protein

被引：15

作者：

Bonsma, S

Gallus, J

Könz, F

Purchase, R

Völker, S

机构：

[1] Leiden Univ, Huygens Lab, NL-2300 RA Leiden, Netherlands

[2] Leiden Univ, Gorlaeus Labs, NL-2300 RA Leiden, Netherlands

来源：

JOURNAL OF LUMINESCENCE | 2004年 / 107卷 / 1-4期

关键词：

green and red fluorescent proteins; photo-induced conformational changes; spectral hole burning; energy transfer; laser excitation and emission spectroscopy;

D O I：

10.1016/j.jlumin.2003.12.044

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Reversible conformational changes have been photo-induced in the red fluorescent protein DsRed at low temperature by wavelength-selective laser irradiation. We have found two new fluorescent forms: a shifted-red (SR-) and a new green (G'-) form that absorb and emit, respectively, similar to14 nm to the red and similar to80 nm to the blue of the 'mature' red (R-) form present in an un-illuminated sample of DsRed. Further, we have identified the 0-0 transitions of the various forms by spectral hole burning and estimated their ground-state energy differences and barrier heights by means of temperature-dependent excitation and fluorescence spectroscopy between 1.6 and 295 K. We have also proven that 'downhill' energy transfer takes place between these forms within the tetrameric structure of DsRed. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：203 / 212

页数：10

共 27 条

[1] Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral [J].