Expression of tandem invertase genes associated with sexual and vegetative growth cycles in potato

被引:49
作者
Maddison, AL [1 ]
Hedley, PE [1 ]
Meyer, RC [1 ]
Aziz, N [1 ]
Davidson, D [1 ]
Machray, GC [1 ]
机构
[1] Scottish Crop Res Inst, Dept Cell & Mol Genet, Dundee DD2 5DA, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
axial bud; beta-fructofuranosidase; pollen; promoter; Solanum tuberosum;
D O I
10.1023/A:1006389013179
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The organisation of two invertase genes (invGE and invGF) linked in direct tandem repeat within the potato genome is detailed. The genes exhibit a similar intron/exon structure which differs from previously described plant invertase genes; while intron locations are conserved between the genes, minor differences in exon length are seen. Both genes encode enzymes with putative extracellular location. Biochemical analysis of gene expression showed expression in floral tissues for both genes, with expression of the upstream gene (invGE) also detected in leaf tissue. Promoter sequences from both genes have been fused to the beta-glucuronidase (GUS) reporter gene (uidA) and transformed into potato. One promoter-GUS reporter construct was also transformed into tobacco. Histochemical analysis of transgenic lines defined specific expression from the downstream (invGF) promoter in potato and tobacco pollen, with expression first detected in the late uninucleate stage of tobacco microspore development. The invGE promoter determined expression in pollen and other floral tissues, but also at lateral nodes in stem, root and tuber. An association of invertase expression with generative tissue, both in vegetative and sexual modes of growth, is indicated.
引用
收藏
页码:741 / 751
页数:11
相关论文
共 48 条
  • [11] A TOBACCO CDNA CODING FOR CELL-WALL INVERTASE
    GREINER, S
    WEIL, M
    KRAUSGRILL, S
    RAUSCH, T
    [J]. PLANT PHYSIOLOGY, 1995, 108 (02) : 825 - 826
  • [12] THE MYB-HOMOLOGOUS P-GENE CONTROLS PHLOBAPHENE PIGMENTATION IN MAIZE FLORAL ORGANS BY DIRECTLY ACTIVATING A FLAVONOID BIOSYNTHETIC GENE SUBSET
    GROTEWOLD, E
    DRUMMOND, BJ
    BOWEN, B
    PETERSON, T
    [J]. CELL, 1994, 76 (03) : 543 - 553
  • [13] THE U6 SMALL NUCLEAR-RNA GENE FAMILY OF POTATO
    GUERINEAU, F
    WAUGH, R
    [J]. PLANT MOLECULAR BIOLOGY, 1993, 22 (05) : 807 - 818
  • [14] Characterization of two members of the Arabidopsis thaliana gene family, At beta fruct3 and At beta fruct4 coding for vacuolar invertases
    HaouazineTakvorian, N
    TymowskaLalanne, Z
    Takvorian, A
    Tregear, J
    Lejeune, B
    Lecharny, A
    Kreis, M
    [J]. GENE, 1997, 197 (1-2) : 239 - 251
  • [15] CDNA CLONING AND EXPRESSION OF A POTATO (SOLANUM-TUBEROSUM) INVERTASE
    HEDLEY, PE
    MACHRAY, GC
    DAVIES, HV
    BURCH, L
    WAUGH, R
    [J]. PLANT MOLECULAR BIOLOGY, 1993, 22 (05) : 917 - 922
  • [16] POTATO (SOLANUM-TUBEROSUM) INVERTASE-ENCODING CDNAS AND THEIR DIFFERENTIAL EXPRESSION
    HEDLEY, PE
    MACHRAY, GC
    DAVIES, HV
    BURCH, L
    WAUGH, R
    [J]. GENE, 1994, 145 (02) : 211 - 214
  • [17] Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL
    Heinemeyer, T
    Wingender, E
    Reuter, I
    Hermjakob, H
    Kel, AE
    Kel, OV
    Ignatieva, EV
    Ananko, EA
    Podkolodnaya, OA
    Kolpakov, FA
    Podkolodny, NL
    Kolchanov, NA
    [J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (01) : 362 - 367
  • [18] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS
    HORSCH, RB
    FRY, JE
    HOFFMANN, NL
    EICHHOLTZ, D
    ROGERS, SG
    FRALEY, RT
    [J]. SCIENCE, 1985, 227 (4691) : 1229 - 1231
  • [19] ISOLATION AND CHARACTERIZATION OF THE BINDING SEQUENCES FOR THE PRODUCT OF THE ARABIDOPSIS FLORAL HOMEOTIC GENE AGAMOUS
    HUANG, H
    MIZUKAMI, Y
    HU, Y
    MA, H
    [J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (20) : 4769 - 4776
  • [20] AN EFFICIENT GENOTYPE-INDEPENDENT METHOD FOR REGENERATION OF POTATO PLANTS FROM LEAF TISSUE
    HULME, JS
    HIGGINS, ES
    SHIELDS, R
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 1992, 31 (02) : 161 - 167