Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction

被引：29

作者：

Chassagne, Laurent ^{[2
]}

Pradel, Nathalle ^{[1
]}

Robin, Frederic ^{[1
,2
]}

Livrelli, Valerie ^{[1
]}

Bonnet, Richard ^{[1
,2
]}

Delmas, Julien ^{[1
,2
]}

机构：

[1] Univ Auvergne Clermont 1, Fac Med Pharm, Bacteriol Lab, JE2526,USC INRA 2018, F-63001 Clermont Ferrand, France

[2] CHU Clermont Ferrand, Ctr Biol, Lab Bacteriol Clin, F-63003 Clermont Ferrand, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2009年 / 64卷 / 01期

关键词：

EHEC; STEC; Real-time; PCR; Stools; SYBR Green; stx; eae; MULTIPLEX PCR ASSAYS; SHIGA TOXIN; STRAINS; O157-H7; IDENTIFICATION; VARIANTS; AMPLIFICATION; MUTATION; INTIMIN; FRANCE;

D O I：

10.1016/j.diagmicrobio.2009.01.031

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We report a multiplex real-time polymerase chain reaction method for detecting enterohemorrhagic Escherichia coli (EHEC) from strains or stool specimens. This assay detected the virulence genes str1, stx2, and eae, without the use of probes. The method, which was validated on a collection of 143 EHEC strains, is simple, rapid, cost-effective, and sensitive. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：98 / 101

页数：4

共 20 条

[1]

CAPRIOLI A, 1995, J MED MICROBIOL, V43, P48

[2] SIMULTANEOUS IDENTIFICATION OF STRAINS OF ESCHERICHIA-COLI SEROTYPE O157-H7 AND THEIR SHIGA-LIKE TOXIN TYPE BY MISMATCH AMPLIFICATION MUTATION ASSAY-MULTIPLEX PCR [J].

CEBULA, TA ;

PAYNE, WL ;

FENG, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :248-250

[3] Clinical Escherichia coli strains carrying stx genes:: stx variants and stx-positive virulence profiles [J].

Eklund, M ;

Leino, K ;

Siitonen, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) :4585-4593

[4] Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypes [J].

Feng, P ;

Monday, SR .

MOLECULAR AND CELLULAR PROBES, 2000, 14 (06) :333-337

[5] Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli O157:H7 [J].

Fortin, NY ;

Mulchandani, A ;

Chen, W .

ANALYTICAL BIOCHEMISTRY, 2001, 289 (02) :281-288

[6] Escherichia coli harboring shiga toxin 2 gene variants:: frequency and association with clinical symptoms [J].

Friedrich, AW ;

Bielaszewska, M ;

Zhang, WL ;

Pulz, M ;

Kuczius, T ;

Ammon, A ;

Karch, H .

JOURNAL OF INFECTIOUS DISEASES, 2002, 185 (01) :74-84

[7] Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains [J].

Gannon, VPJ ;

DSouza, S ;

Graham, T ;

King, RK ;

Rahn, K ;

Read, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (03) :656-662

[8] Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR [J].

Guion, Chase E. ;

Ochoa, Theresa J. ;

Walker, Christopher M. ;

Barletta, Francesca ;

Cleary, Thomas G. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (05) :1752-1757

[9]

Oberst RD, 1998, APPL ENVIRON MICROB, V64, P3389

[10] Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E-coli hlyA, rfbO111, and rfbO157 [J].

Paton, AW ;

Paton, JC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (02) :598-602

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