A study on the molecular mechanism of resistance to amicarthiazol in Xanthomonas campestris pv. citri

被引：31

作者：

Li, J ^{[1
]}

Zhou, MG ^{[1
]}

Li, HX ^{[1
]}

Chen, CJ ^{[1
]}

Wang, JX ^{[1
]}

Zhang, YJ ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

来源：

PEST MANAGEMENT SCIENCE | 2006年 / 62卷 / 05期

关键词：

Xanthomonas campestris pv. citri; amicarthiazol; resistance mechanism; succinate dehydrogenase;

D O I：

10.1002/ps.1187

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Three amicarthiazol-resistant mutants (Xuv10, Xuv20 and Xuv40) were obtained by UV induction and used in this study. Minimal inhibition concentrations (MICs) of amicarthiazol against the growth of mutants and wild-type isolate were 400 and 100 mu g ml(-1) respectively. Inhibition by amicarthiazol of succinate dehydrogenase (SDH) activities of Xanthomonas campestris pv. citri (Hasse) Dye wild-type isolate (Xcc) and three resistant mutants derived from this isolate were assayed using triphenyltetrazolium. chloride (TTC). The SDH activities of these mutants were significantly lower than that of Xcc. The complete nucleotide sequences of four subunits (SdhA, SdhB, SdhC and SdhD) of succinate-ubiquinone oxidoreductase (SQR) were cloned by polymerase chain reaction (PCR) amplification. An amino acid mutation (HiS(229) -> Leu(229)) in sdhB was found to confer resistance of X. campestris pv. citri to amicarthiazol. It is suggested that this mutation alters the SDH complex in some way that prevents binding of amicarthiazol. (c) 2006 Society of Chemical Industry.

引用

页码：440 / 445

页数：6

共 24 条

[1]

AKRELL BAC, 1992, CHEM BIOCH FLAVOENZY, V3, P229

[2]

Ausubel F.M., 1994, CURRENT PROTOCOLS MO

[3] A SINGLE AMINO-ACID CHANGE IN THE IRON-SULFUR PROTEIN SUBUNIT OF SUCCINATE-DEHYDROGENASE CONFERS RESISTANCE TO CARBOXIN IN USTILAGO-MAYDIS [J].

BROOMFIELD, PLE ;

HARGREAVES, JA .

CURRENT GENETICS, 1992, 22 (02) :117-121

[4] Succinate dehydrogenase and fumarate reductase from Escherichia coli [J].

Cecchini, G ;

Schröder, I ;

Gunsalus, RP ;

Maklashina, E .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2002, 1553 (1-2) :140-157

[5]

CHUNMING X, 1991, JIANGSU PESTIC, V3, P29

[6]

Dye D. W., 1980, Review of Plant Pathology, V59, P153

[7]

HARGERHALL, 1995, BIOCHIM BIOPHYS ACTA, V1229, P356

[8]

HARRISON WA, 1973, Patent No. 3709992

[9]

HARRISON WA, 1971, Patent No. 873888

[10]

HARRISON WA, 1970, Patent No. 3547917

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