Chemiluminescence quenching of pteroic acid-N-sulfonyl-acridinium-9-carboxamide conjugates by folate binding protein

被引：12

作者：

Adamczyk, M ^{[1
]}

Fino, JR ^{[1
]}

Mattingly, PG ^{[1
]}

Moore, JA ^{[1
]}

Pan, Y ^{[1
]}

机构：

[1] Abbott Labs, Dept Chem D09MD, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 09期

关键词：

homogeneous chemiluminescent assays; folate binding protein;

D O I：

10.1016/j.bmcl.2004.01.105

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

N-10-Trifluoroacetylpteroic acid was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamide labels at the N-10 or 9-position carboxamide. Upon binding to folate binding protein the light output of the N-10 derivative (9) was quenched up to 62% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (7) was quenched only 12%. The utility of this effect was demonstrated in a model homogeneous cherm luminescent assay for folic acid. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：2313 / 2317

页数：5

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