Regiodependent luminescence quenching of biotinylated N-sulfonyl-acridinium-9-carboxamides by avidin

被引：11

作者：

Adamczyk, M ^{[1
]}

Mattingly, PG ^{[1
]}

Moore, JA ^{[1
]}

Pan, Y ^{[1
]}

机构：

[1] Abbott Labs, Div Diagnost, Dept Chem D09MD, Abbott Pk, IL 60064 USA

来源：

ORGANIC LETTERS | 2003年 / 5卷 / 21期

关键词：

D O I：

10.1021/ol035323p

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] Biotin was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxantides at the N-10 or 9-position carboxamide. Upon binding to avidin, the light output of the N-10 derivative (8) was quenched up to 92% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (9) was quenched only 33%. The utility of this effect was demonstrated in a model homogeneous chemiluminescence assay.

引用

页码：3779 / 3782

页数：4

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