DNA aptamer-based detection of lysozyme by an electrochemiluminescence assay coupled to quantum dots

被引：92

作者：

Huang, Haiping ^{[1
]}

Jie, Guifen ^{[1
]}

Cui, Rongjing ^{[1
]}

Zhu, Jun-Jie ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci MOE, Nanjing 210093, Peoples R China

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2009年 / 11卷 / 04期

基金：

中国国家自然科学基金;

关键词：

DNA; Electrochemiluminescence; Quantum dots; Lysozyme; Biosensors; ELECTROGENERATED CHEMILUMINESCENCE; NANOCRYSTALS; BIOSENSOR; ELECTROCHEMISTRY; NANOPARTICLES; MOLECULES; PROTEINS;

D O I：

10.1016/j.elecom.2009.01.009

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A novel quantum dots (QDs) ECL biosensor for the detection of lysozyme was developed. Lysozyme was first incubated with probes immobilized at Au electrode in order to form the aptamer-lysozyme bioaffinity complexes. And the free probes were hybridized with the 5'-biotin modified cDNA oligonucleotides to form double-stranded DNA (ds-DNA) oligonucleotides. Avidin-QDs were bound to these hybridized cDNA through the biotin-avidin-system. The ECL signal of the biosensor was responsive to the amount of QDs bonded to the cDNA oligonucleotides, which was indirectly inverse proportional to the combined target protein. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：816 / 818

页数：3

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