Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp AH-101 as Bacillus halodurans

被引：50

作者：

Takami, H ^{[1
]}

Nogi, Y ^{[1
]}

Horikoshi, K ^{[1
]}

机构：

[1] Japan Marine Sci & Technol Ctr, Deep Sea Microorganisms Res Grp, Yokosuka, Kanagawa 2370061, Japan

来源：

EXTREMOPHILES | 1999年 / 3卷 / 04期

关键词：

alkaliphilic; Bacillus halodurans; AH-101; DNA-DNA hybridization; phylogenetic tree; identification;

D O I：

10.1007/s007920050130

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alkaliphilic Bacillus sp. AH-101 was characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA-DNA hybridization analyses were performed. Phylogenetic analysis of strain AH-101 based on comparison of 16S rDNA sequences revealed that this strain is closely related to Bacillus halodurans. DNA-DNA hybridization of AH-101 and related Bacillus reference strains showed that the highest level of DNA-DNA relatedness (88%) was found between strain AH-101 and the B. halodurans type strain (DSM497). Our findings demonstrate that strain AH-101 is a member of the species B. halodurans.

引用

页码：293 / 296

页数：4

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