Species differences in localization of cardiac cAMP-phosphodiesterase activity: A cytochemical study

被引：4

作者：

Okruhlicova, L ^{[1
]}

Tribulova, N ^{[1
]}

Styk, J ^{[1
]}

Eckly, A ^{[1
]}

Lugnier, C ^{[1
]}

Slezak, J ^{[1
]}

机构：

[1] UNIV LOUIS PASTEUR STRASBOURG 1, LAB PHARMACOL & PHYSIOL CELLULAIRE,CNRS, F-67401 ILLKIRCH GRAFFENSTADEN, FRANCE

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1997年 / 173卷 / 1-2期

关键词：

phosphodiesterase; cytochemistry; myocardium; rat; dog;

D O I：

10.1023/A:1006845513962

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The localization of the membrane-bound cyclic 3',5'-AMP phosphodiesterase in cardiac tissues of both, rat and dog was studied by cytochemical method. 40 mu m thick slices from glutaraldehyde fixed heart tissue were incubated in the medium with cAMP as a substrate and Pb ions as a capture metal of the reaction product. The cAMP-PDE activity in the rat ventricle was only shown positive on the sarcolemma. Whereas, in canine ventricular tissue the cAMP-PDE activity in cardiomyocytes was shown on the sarcolemma, on the junctional sarcoplasmic reticulum and on subsarcolemmal cisternae. The results confirm differences in the localization of cAMP-PDE in dog and rat heart.

引用

页码：183 / 188

页数：6

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