A two-dimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining

被引：43

作者：

Hayduk, EJ ^{[1
]}

Choe, LH ^{[1
]}

Lee, KH ^{[1
]}

机构：

[1] Cornell Univ, Sch Chem & Biomol Engn, Ithaca, NY 14850 USA

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 15期

关键词：

Chinese hamster ovary cells; pro-Q diamond; proteomics; SYPRO Ruby; tandem time-of-flight;

D O I：

10.1002/elps.200406010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the development of a detailed two-dimensional electrophoresis map of Chinese hamster ovary (CHO) cell proteins based on fluorescence staining and tandem time-of-flight (TOF/TOF)-mass spectrometry. We observed a 71% success rate in the identification of proteins even though the CHO genome is not sequenced. The map consists of 224 protein identifications present in 274 two-dimensional gel electrophoresis (2-DE) gel spots. We have also initiated a study of the phosphoproteome using a commercially available phosphoprotein-specific fluorescent stain. Using this stain, we observe 672 phosphorylated proteins, including many proteins known to be phosphorylated, which is 36% of the proteins we visualized with a total protein stain and consistent with expectations.

引用

页码：2545 / 2556

页数：12

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