Sequencing and analysis of 14,842 expressed sequence tags of burma mangrove, Bruguiera gymnorrhiza

被引：31

作者：

Miyama, Masashi

Shimizu, Hidetsugu

Sugiyama, Masahide

Hanagata, Nobutaka

机构：

[1] Natl Inst Marine Sci, Biomat Ctr, Tsukuba, Ibaraki 3050044, Japan

[2] Tokyo Univ Technol, Sch Bion, Hachioji, Tokyo 1920982, Japan

[3] Hitachi Instruments Serv Co Ltd, Biosyst Business Dept, Shinjyuku Ku, Tokyo 1600004, Japan

来源：

PLANT SCIENCE | 2006年 / 171卷 / 02期

关键词：

expressed sequence tags; burma mangrove; salt tolerance; gene expression;

D O I：

10.1016/j.plantsci.2006.03.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here sequencing and analysis of expressed sequence tags (ESTs) generated from burma mangrove, Bruguiera gymnorrhiza. A total of 26,400 cDNA were partially sequenced from five different cDNA libraries constructed from the leaves and roots. These sequences were processed into 14,842 high-quality sequences. Clustering and assembling of these sequences resulted in 6943 unique genes. Blast searches show that 4339 (62.5%) genes had a similarity with known proteins in UniProt database. Of these, 2074 genes are assigned in at least one Gene Ontology terms. Expression profiles are generated based on EST frequency in each cDNA library. The profile suggested co-expression and organ specific gene regulations. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

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收藏

页码：234 / 241

页数：8

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