Comparative study of morphological, cultural and molecular markers for the characterization of Pseudocercosporella herpotrichoides isolates

被引：12

作者：

Gac, ML ^{[1
]}

Montfort, F ^{[1
]}

Cavelier, N ^{[1
]}

Sailland, A ^{[1
]}

机构：

[1] RHONE POULENC AGRO,BIOTECHNOL LAB,F-69009 LYON,FRANCE

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 1996年 / 102卷 / 04期

关键词：

eyespot; internal transcribed spacer; polymerase chain reaction; restriction analysis; ribosomal RNA genes;

D O I：

10.1007/BF01878127

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Nirenberg's classification system and the polymerase chain reaction (PCR) combined with restriction enzyme digestion of an amplified ribosomal DNA fragment, were compared for the characterization of sixty isolates of Pseudocercosporella herpotrichoides, from various geographical areas and with differing fungicide sensitivity. With Nirenberg's system, it was possible to identify most isolates as P. herpotrichoides var. herpotrichoides or P. herpotrichoides var. acuformis. However, identification was slow and sometimes inconclusive as overlap occurred between the two varieties for all criteria examined. Molecular markers identified two distinct types among the isolates tested and generally good correlation was found between the PCR-based assay and Nirenberg's system, but the molecular assay was more accurate and faster.

引用

页码：325 / 337

页数：13