Efficient delivery of anticancer drug MTX through MTX-LDH nanohybrid system

被引:163
作者
Oh, Jae-Min
Park, Man
Kim, Sang-Tae
Jung, Jin-Young
Kang, Yong-Gu
Choy, Jin-Ho [1 ]
机构
[1] Ewha Womans Univ, Div Nanosci, Ctr Intelligent NanoBio Mat, Seoul 120750, South Korea
[2] Ewha Womans Univ, Dept Chem, Seoul 120750, South Korea
[3] Catholic Univ, Coll Med, Dept Orthopaed Surg, Inst Clin Med, Suwon 442060, South Korea
关键词
D O I
10.1016/j.jpcs.2006.01.033
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We have been successful to intercalate anticancer drug, methotrexate (MTX), into layered double hydroxides (LDHs), Mg2Al(OH)(6) (NO3) center dot 0.1H(2)O, through conventional co-precipitation method. Layered double hydroxides (LDHs) are endowed with great potential for delivery vector, since their cationic layers lead to safe reservation of biofunctional molecules such as drug molecules or genes. And their ion exchangeability and solubility in acidic media (pH < 4) give rise to the controlled release of drug molecules. Moreover, it has been partly confirmed that LDH itself is non-toxic and facilitate the cellular permeation. To check the toxicity of LDHs, the osteosarcoma cell culture lines (Saos-2 and MG-63) and the normal one (human fibroblast) were used for in vitro test. The anticancer efficacy of MTX intercalated LDHs (MTXLDH nanohybrids) was also estimated in vitro by the bioassay such as MTT and BrdU (5-bromo-2-deoxyuridine) with the bone cancer cell culture lines (Saos-2 and MG-63). According to the toxicity test results, LDHs do not harm to both the normal and cancer cells upto the concentration of 500 ug/mL. The anticancer efficacy test for the MTX-LDH nanohybrids turn out to be much more effective in cell suppression compared to the MTX itself. According to the cell-line tests, the MTX-LDH shows same drug efficacy to the MTX itself in spite of the low concentration by similar to 5000 times. Such a high cancer suppression effect of MTX-LDH hybrid is surely due to the excellent delivery efficiency of inorganic delivery vector, LDHs. (c) 2006 Elsevier Ltd. All rights reserved.
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收藏
页码:1024 / 1027
页数:4
相关论文
共 11 条
[1]  
BAILEY LB, 1995, FOLATE HLTH DIS
[2]  
Choy JH, 2000, ANGEW CHEM INT EDIT, V39, P4042
[3]   Inorganic-biomolecular hybrid nanomaterials as a genetic molecular code system [J].
Choy, JH ;
Oh, JM ;
Park, M ;
Sohn, KM ;
Kim, JW .
ADVANCED MATERIALS, 2004, 16 (14) :1181-+
[4]   Intercalative route to heterostructured nanohybrids [J].
Choy, JH ;
Paek, SM ;
Oh, JM ;
Jang, ES .
CURRENT APPLIED PHYSICS, 2002, 2 (06) :489-495
[5]   Layered double hydroxide as an efficient drug reservoir for folate derivatives [J].
Choy, JH ;
Jung, JS ;
Oh, JM ;
Park, M ;
Jeong, J ;
Kang, YK ;
Han, OJ .
BIOMATERIALS, 2004, 25 (15) :3059-3064
[6]  
GILMAN AG, PHARM BASIS THERAPEU
[7]  
KUMAR HP, 1987, J BIOL CHEM, V262, P7171
[8]   Molecular and cellular biology of the human reduced folate carrier [J].
Matherly, LH .
PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 67, 2001, 67 :131-162
[9]  
SHIN DS, 1998, J KOREAN BONE JOINT, V4, P13
[10]   COMPARISON OF TRANSPORT-PROPERTIES OF THE REDUCED FOLATE CARRIER AND FOLATE RECEPTOR IN MURINE L1210 LEUKEMIA-CELLS [J].
SIERRA, EE ;
BRIGLE, KE ;
SPINELLA, MJ ;
GOLDMAN, ID .
BIOCHEMICAL PHARMACOLOGY, 1995, 50 (08) :1287-1294