C19MC microRNAs are processed from introns of large Pol-II, non-protein-coding transcripts

被引:186
作者
Bortolin-Cavaille, Marie-Line [1 ,2 ]
Dance, Marie [1 ,2 ]
Weber, Michel [1 ,2 ]
Cavaille, Jerome [1 ,2 ]
机构
[1] Univ Toulouse, UPS, Lab Biol Mol Eucaryote, F-31000 Toulouse, France
[2] LBME, CNRS, F-31000 Toulouse, France
关键词
GENES; RNAS; IDENTIFICATION; EXPRESSION; CLUSTER; BIOGENESIS; INHIBITORS; EVOLUTION; COMPLEX; STRESS;
D O I
10.1093/nar/gkp205
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
MicroRNAs are tiny RNA molecules that play important regulatory roles in a broad range of developmental, physiological or pathological processes. Despite recent progress in our understanding of miRNA processing and biological functions, little is known about the regulatory mechanisms that control their expression at the transcriptional level. C19MC is the largest human microRNA gene cluster discovered to date. This 100-kb long cluster consists of 46 tandemly repeated, primate-specific pre-miRNA genes that are flanked by Alu elements (Alus) and embedded within a 400- to 700-nt long repeated unit. It has been proposed that C19MC miRNA genes are transcribed by RNA polymerase III (Pol-III) initiating from A and Bboxesembedded in upstream Alu repeats. Here, we show that C19MC miRNAs are intron-encoded and processed by the DGCR8-Drosha (Microprocessor) complex from a previously unidentified, non-protein-coding Pol-II (and not Pol-III) transcript which is mainly, if not exclusively, expressed in the placenta.
引用
收藏
页码:3464 / 3473
页数:10
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