Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses

被引：79

作者：

Boanini, Elisa ^{[1
]}

Torricelli, Paola ^{[2
,3
]}

Gazzano, Massimo ^{[4
]}

Della Bella, Elena ^{[2
]}

Fini, Milena ^{[2
,3
]}

Bigi, Adriana ^{[1
]}

机构：

[1] Univ Bologna, Dept Chem G Ciamician, Bologna, Italy

[2] Rizzoli Orthopaed Inst, Codivilla Putti Res Inst, Laboratoiy Preclin & Surg Studies, Bologna, Italy

[3] Rizzoli Orthopaed Inst IOR, Dept Rizzoli RIT, Lab Biocompatibil Technol Innovat & Adv Therapies, Bologna, Italy

[4] CNR, ISOF, Dept Chem G Ciamician, I-40126 Bologna, Italy

来源：

BIOMATERIALS | 2014年 / 35卷 / 21期

关键词：

Strontium; Bisphosphonates; Hydroxyapatite; Osteoblast; Osteoclast; Co-cultures; NITROGEN-CONTAINING BISPHOSPHONATES; OSTEOBLAST-LIKE CELLS; IN-VITRO; POSTMENOPAUSAL OSTEOPOROSIS; OSTEOCLAST FORMATION; RANELATE TREATMENT; ACID; INHIBIT; DIFFERENTIATION; MINERALIZATION;

D O I：

10.1016/j.biomaterials.2014.03.053

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

The influence of the simultaneous presence of the two inhibitors of bone degradation, strontium and zoledronate, on the direct synthesis of hydroxyapatite was explored in the range of Sr concentration up to 50 atom% at two different bisphosphonate concentrations (ZOL7 and ZOL14). The results of structural analysis indicated that HA can be obtained as a single crystalline phase up to a Sr concentration in solution of 20 and 10 atom% within the ZOL7 and ZOL14 series respectively. Both Sr substitution and ZOL incorporation affect the length of the coherently scattering crystalline domains and the dimensions of HA nanocrystals. At greater Sr content, XRD full profile fitting data indicate that zoledronate provokes the segregation of Sr in two crystalline apatitic phases, at different strontium content. Co-cultures of osteoblast-like MG63 cells and human osteoclast show that ZOL displays a greater inhibitory influence than Sr on osteoclast proliferation and activity. On the other hand, the results obtained on osteoblast surnatant and on gene expression indicate that Sr exerts a greater promotion on osteoblast proliferation and differentiation. The co-presence of Sr and ZOL has a combined effect on the differentiation markers, so that HA containing about 4 wt% ZOL and 4 Sr atom%, and even more HA containing about 4 wt% ZOL and 8 Sr atom%, result the best compromise for osteoblast promotion and osteoclast inhibition. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：5619 / 5626

页数：8

共 46 条

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Strontium ranelate treatment of human primary osteoblasts promotes an osteocyte-like phenotype while eliciting an osteoprotegerin response [J].