Investigation of selenium tolerance mechanisms in Arabidopsis thaliana

被引:30
作者
Zhang, Li-Hong [1 ]
Abdel-Ghany, Salah E. [1 ]
Freeman, John L. [1 ]
Ackley, Ashley R. [1 ]
Schiavon, Michela [1 ]
Pilon-Smits, Elizabeth A. H. [1 ]
机构
[1] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA
关键词
D O I
10.1111/j.1399-3054.2006.00739.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To investigate selenium tolerance mechanisms in Arabidopsis thaliana, genetic and physiologic studies were performed in the three Arabidopsis accessions Landsberg erecta (Ler), Columbia (Col) and Wassilewskija (Ws). Accession Ler was significantly less tolerant to selenate than Ws and Col, whereas Ws was less tolerant to selenite than the others. Analysis of selenium tolerance in F-1 and F-2 plants obtained from crosses between these accessions suggest that multiple genes are involved in selenate tolerance and a single major gene controls selenite tolerance in these populations. Bulked segregant analysis in two F-2 populations indicated that molecular marker ciw7 on chromosome 4 is linked to selenite tolerance, and three molecular markers on chromosomes 1, 3 and 5 (nga111, ciw4 and ciw8, respectively) are linked to selenate tolerance. The ecotypic variation in selenite tolerance appeared to be correlated with root levels of non-protein thiols. Also, the shoot tissue levels of selenocysteine (SeCys) and selenocystine were correlated with tolerance to both selenate and selenite. Judging from RT-PCR results, several sulfate transporters and S assimilatory enzymes appear to be upregulated by selenate and selenite at the transcriptional level. A potential SeCys methyltransferase was expressed at lower levels in selenite-sensitive Ws than in the other two, when grown on selenite. Together, these studies show that there is substantial intraspecific variation in tolerance to selenate and selenite in Arabidopsis, and provide insights into the genetic and biochemical mechanisms underlying the observed ecotypic differences.
引用
收藏
页码:212 / 223
页数:12
相关论文
共 44 条
[1]  
ANDERSON JW, 1993, SULFUR NUTRITION AND ASSIMILATION IN HIGHER PLANTS, P49
[2]   ASSIGNMENT OF 30 MICROSATELLITE LOCI TO THE LINKAGE MAP OF ARABIDOPSIS [J].
BELL, CJ ;
ECKER, JR .
GENOMICS, 1994, 19 (01) :137-144
[3]   Trends in selenium biochemistry [J].
Birringer, M ;
Pilawa, S ;
Flohé, L .
NATURAL PRODUCT REPORTS, 2002, 19 (06) :693-718
[4]   SELENIUM METABOLISM IN NEPTUNIA-AMPLEXICAULIS [J].
BURNELL, JN .
PLANT PHYSIOLOGY, 1981, 67 (02) :316-324
[5]  
BYERS HG, 1936, USDA AGR TECHN B, V530
[6]   The glutathione-deficient, cadmium-sensitive mutant, cad2-1, of Arabidopsis thaliana is deficient in γ-glutamylcysteine synthetase. [J].
Cobbett, CS ;
May, MJ ;
Howden, R ;
Rolls, B .
PLANT JOURNAL, 1998, 16 (01) :73-78
[7]   Phytochelatins and their roles in heavy metal detoxification [J].
Cobbett, CS .
PLANT PHYSIOLOGY, 2000, 123 (03) :825-832
[8]   Rate-limiting steps in selenium assimilation and volatilization by Indian mustard [J].
de Souza, MP ;
Pilon-Smits, EAH ;
Lytle, CM ;
Hwang, S ;
Tai, J ;
Honma, TSU ;
Yeh, L ;
Terry, N .
PLANT PHYSIOLOGY, 1998, 117 (04) :1487-1494
[9]   ACTIVATION OF SELENATE BY ADENOSINE 5'-TRIPHOSPHATE SULFURYLASE FROM SACCHAROMYCES-CEREVISIAE [J].
DILWORTH, GL ;
BANDURSKI, RS .
BIOCHEMICAL JOURNAL, 1977, 163 (03) :521-529
[10]   Production of Se-methylselenocysteine in transgenic plants expressing selenocysteine methyltransferase [J].
Ellis D.R. ;
Sors T.G. ;
Brunk D.G. ;
Albrecht C. ;
Orser C. ;
Lahner B. ;
Wood K.V. ;
Harris H.H. ;
Pickering I.J. ;
Salt D.E. .
BMC Plant Biology, 4 (1)