Mushroom body influence on locomotor activity and circadian rhythms in Drosophila melanogaster

被引:15
作者
Helfrich-Förster, C
Wulf, J
de Belle, JS
机构
[1] Univ Nevada, Dept Biol Sci, Las Vegas, NV 89154 USA
[2] Max Planck Inst Biol Cybernet, D-72076 Tubingen, Germany
[3] Inst Bot, D-72076 Tubingen, Germany
[4] Zool Inst Tierphysiol, D-72076 Tubingen, Germany
[5] Max Planck Inst Marine Mikrobiol, D-28359 Bremen, Germany
关键词
corpora pedunculata; hydroxyurea; period gene; mushroom body mutants; pigment-dispersing hormone; immunohistochemistry;
D O I
暂无
中图分类号
Q3 [遗传学];
学科分类号
071007 [遗传学]; 090102 [作物遗传育种];
摘要
Whether or not mechanisms underlying circadian locomotor rhythms and learning are related anatomically through the mushroom bodies (MBs) was investigated by monitoring behavioral rhythmicity in flies with MB lesions induced by chemical ablation and by mutations in five different genes. All flies tested were later examined histologically to assess (1) MB neuroanatomy, and (2) the condition of the putative pacemaker cells - the ventral Lateral Neurons (LN(v)s) and their terminals that project to the vicinity of the MB calyces. All groups of flies had normal rhythms except for mushroom body miniature (mbm; only in a wild-type Berlin genetic background) and mushroom body defect (mud). MB ablation had no effect on the gender-specific differences in the rhythmic activity profile that are typical of wild-type flies. However, ablated males had a slightly longer period than untreated males and were more active under constant dark conditions. LN(v)s and their arborization patterns appeared normal in MB-ablated and in most mutant flies. Activity defects of mbm flies were attributed to genetic background rather than to the mutation alone. Misrouted LNv projections and similar to14% arrhythmia of mud flies were uncorrelated and attributed to pleiotropy rather than to specific effects of MB lesions. Our results imply that MBs are not involved in circadian activity rhythms but that they do have an inhibitory effect on activity levels of male flies.
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页码:73 / +
页数:38
相关论文
共 112 条
[1]
Resetting of the circadian clock by a conditioned stimulus [J].
Amir, S ;
Stewart, J .
NATURE, 1996, 379 (6565) :542-545
[2]
[Anonymous], PROGR ZOOLOGY
[3]
Armstrong JD, 1998, LEARN MEMORY, V5, P102
[4]
Conditioned stimulus control in the circadian system: Two tales tell one story [J].
Arvanitogiannis, A ;
Stewart, J ;
Amir, S .
JOURNAL OF BIOLOGICAL RHYTHMS, 2000, 15 (04) :292-293
[5]
Arvanitogiannis A, 1999, BEHAV NEUROSCI, V113, P1297
[6]
The Drosophila dCREB2 gene affects the circadian clock [J].
Belvin, MP ;
Zhou, H ;
Yin, JCP .
NEURON, 1999, 22 (04) :777-787
[7]
The Drosophila circadian clock:: what we know and what we don't know [J].
Blau, J .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2001, 12 (04) :287-293
[8]
DEFICIENT LONG-TERM-MEMORY IN MICE WITH A TARGETED MUTATION OF THE CAMP-RESPONSIVE ELEMENT-BINDING PROTEIN [J].
BOURTCHULADZE, R ;
FRENGUELLI, B ;
BLENDY, J ;
CIOFFI, D ;
SCHUTZ, G ;
SILVA, AJ .
CELL, 1994, 79 (01) :59-68
[9]
THE RELATIONSHIP BETWEEN LOCOMOTOR-ACTIVITY AND COURTSHIP IN THE MELANOGASTER SPECIES SUBGROUP OF DROSOPHILA [J].
COBB, M ;
CONNOLLY, K ;
BURNET, B .
ANIMAL BEHAVIOUR, 1987, 35 :705-713
[10]
Associative learning disrupted by impaired G(s) signaling in Drosophila mushroom bodies [J].
Connolly, JB ;
Roberts, IJH ;
Armstrong, JD ;
Kaiser, K ;
Forte, M ;
Tully, T ;
OKane, CJ .
SCIENCE, 1996, 274 (5295) :2104-2107