Ratiometric probes for hydrogencarbonate analysis in intracellular or extracellular environments using europium luminescence

被引：74

作者：

Bretonnière, Y

Cann, MJ

Parker, D

Slater, R

机构：

[1] Univ Durham, Dept Chem, Durham DH1 3LE, England

[2] Univ Durham, Sch Biol Sci, Sci Labs, Durham DH1 3LE, England

来源：

CHEMICAL COMMUNICATIONS | 2002年 / 17期

关键词：

D O I：

10.1039/b206286k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A series of six, cationic, zwitterionic and anionic Eu complexes has been examined for the analysis of hydrogen-carbonate concentration in the intracellular and extracellular ranges; an anionic complex incorporating three glutarate residues and a sensitising acridone chromophore (lambda(exc) = 410 nm) exhibits a 69% change in the intensity ratio of the 618/588 nm Eu emission bands between 5 and 15 mM HCO3- in a cell lysate medium.

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页码：1930 / 1931

页数：2

相关论文

共 21 条

[1] Anion recognition by dimetallic cryptates [J].

Amendola, V ;

Fabbrizzi, L ;

Mangano, C ;

Pallavicini, P ;

Poggi, A ;

Taglietti, A .

COORDINATION CHEMISTRY REVIEWS, 2001, 219 :821-837

[2] Functional characterization of a cloned human kidney Na+:HCO3- cotransporter [J].

Amlal, H ;

Wang, ZH ;

Burnham, C ;

Soleimani, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (27) :16810-16815

[3] The efficient intramolecular sensitisation of terbium(III) and europium(III) by benzophenone-containing ligands [J].

Beeby, A ;

Bushby, LM ;

Maffeo, D ;

Williams, JAG .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 2000, (07) :1281-1283

[4] Non-radiative deactivation of the excited states of europium, terbium and ytterbium complexes by proximate energy-matched OH, NH and CH oscillators: an improved luminescence method for establishing solution hydration states [J].

Beeby, A ;

Clarkson, IM ;

Dickins, RS ;

Faulkner, S ;

Parker, D ;

Royle, L ;

de Sousa, AS ;

Williams, JAG ;

Woods, M .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1999, (03) :493-503

[5] Sol gel-immobilised terbium complexes for luminescent sensing of dissolved oxygen by analysis of emission decay [J].

Blair, S ;

Kataky, R ;

Parker, D .

NEW JOURNAL OF CHEMISTRY, 2002, 26 (05) :530-535

[6] Narrow-range optical pH sensors based on luminescent europium and terbium complexes immobilized in a sol gel glass [J].

Blair, S ;

Lowe, MP ;

Mathieu, CE ;

Parker, D ;

Senanayake, PK ;

Kataky, R .

INORGANIC CHEMISTRY, 2001, 40 (23) :5860-5867

[7] The selectivity of reversible oxy-anion binding in aqueous solution at a chiral europium and terbium center: Signaling of carbonate chelation by changes in the form and circular polarization of luminescence emission [J].

Bruce, JI ;

Dickins, RS ;

Govenlock, LJ ;

Gunnlaugsson, T ;

Lopinski, S ;

Lowe, MP ;

Parker, D ;

Peacock, RD ;

Perry, JJB ;

Aime, S ;

Botta, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (40) :9674-9684

[8] Survey of factors determining the circularly polarised luminescence of macrocyclic lanthanide complexes in solution [J].

Bruce, JI ;

Parker, D ;

Lopinski, S ;

Peacock, RD .

CHIRALITY, 2002, 14 (07) :562-567

[9] Experimental assessment of lanthanide ion donor preference:: spectroscopic and theoretical dissection of static charge and dynamic polarisation contributions to axial ligation in a C4-symmetric chiral europium complex [J].

Bruce, JI ;

Parker, D ;

Tozer, DJ .

CHEMICAL COMMUNICATIONS, 2001, (21) :2250-2251

[10] Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor [J].

Chen, YQ ;

Cann, MJ ;

Litvin, TN ;

Iourgenko, V ;

Sinclair, ML ;

Levin, LR ;

Buck, J .

SCIENCE, 2000, 289 (5479) :625-628