Horizontal transfer of parC and gyrA in fluoroquinolone-resistant clinical isolates of Streptococcus pneumoniae

被引：78

作者：

Ferrándiz, MJ

Fenoll, A

Liñares, J

De la Campa, AG ^{[1
]}

机构：

[1] Inst Salud Carlos III, Ctr Nacl Biol Fundamental, CSIC, Unidad Genet Bacteriana, Madrid 28220, Spain

[2] Inst Salud Carlos III, Ctr Nacl Microbiol, Serv Bacteriol, Madrid 28220, Spain

[3] Ciudad Sanitaria Univ Bellvitge, Hosp Princeps Espanya, Microbiol Serv, Barcelona 08907, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2000年 / 44卷 / 04期

关键词：

D O I：

10.1128/AAC.44.4.840-847.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have analyzed genetically three clinical isolates (3180, 3870, and 1244) of Streptococcus pneumoniae with high-level ciprofloxacin resistance. Isolates 3180 and 3870 were atypical because of their insolubility in deoxycholate. However, they hybridized specifically with pneumococcal autolysin and pneumolysin gene probes and have typical pneumococcal atpC and atpA gene sequences. Analysis of the complete sequences of the parC and gyrA genes revealed total variations of 8 and 8.7% (isolate 3180) and 7.4 and 3.6% (isolate 3870), respectively, compared to the wild-type strain R6 sequence. The variations observed between the sequences of R6 and isolate 1244 were less than 0.9%. The structure of the gyrA and parC genes from isolates 3180 and 3870 was organized in sequence blocks that shown different levels of divergence, suggesting a pattern of recombination. These results are evidence for recombination at the fluoroquinolone target genes in clinical isolates of S. pneumoniae. The genetically related viridans group streptococci could act as a reservoir for fluoroquiuolone resistance genes.

引用

页码：840 / 847

页数：8

共 53 条

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